摘要
目的 研究不同浓度枸杞多糖对小鼠胰岛β细胞系MIN6细胞增殖活性和凋亡的影响.方法 培养MIN6细胞,分为对照组、不同浓度枸杞多糖干预组,分别给予0,100,200,400 mg/L枸杞多糖干预24 h.噻唑蓝（MTT）法检测MIN6细胞增殖活性,流式细胞术检测MIN6细胞凋亡率,Western印迹分析磷酸化细胞外信号调节激酶（ERK）及B淋巴细胞瘤-2（Bcl-2）蛋白表达.结果 与对照组相比,100、200 mg/L枸杞多糖干预组MIN6细胞增殖显著增加（P均＜0.01）,MIN6细胞凋亡显著受抑制（P均＜0.01）,而400 mg/L枸杞多糖干预组细胞增殖活性降低（P＜0.05）,MIN6细胞凋亡增加（P＜0.01）.Western印迹显示,与对照组相比,100,200,400 mg/L枸杞多糖干预组Bcl-2表达（F=65.26,P＜0.01）及磷酸化ERK水平（F=14.85,P＜0.01）均增加,而400 mg/L枸杞多糖干预组Bcl-2与磷酸化ERK表达下降（P均＜0.05）,200 mg/L枸杞多糖干预组与400 mg/L枸杞多糖干预组相比,Bcl-2与磷酸化ERK水平差异均有统计学意义（P均＜0.01）.结论 低浓度枸杞多糖可促进MIN6细胞增殖,减少MIN6细胞凋亡,而高浓度枸杞多糖作用相反,其机制可能与ERK信号通路有关.
Objective To study the effects of different concentration lycium barbarum polysaccharide （LBP） on mouse islet β cell line MIN6 cells viability and apoptosis.Methods MIN6 cells were cultured and divided into control group and different concentration LBP intervene group,treated separately with 0,100,200,400 mg/L LBP for 24 h.Cells viability and apoptosis were separately detected by 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay and flow cytometry.The expression of phosphorylated extracellular regulated protein kinase （ERK） and B-cell lymphoma 2 （Bcl-2） were analyzed by Western blot.Results Compared with control group,MIN6 cells viability was promoted （all P ＜ 0.01）,apoptosis was repressed （all P ＜0.01） in 100,200 mg/L LBP intervene group,while MIN6 cells viability was decreased （P ＜ 0.05） and apoptosis was increased （P ＜ 0.05） in 400 mg/L LBP intervene group.Compared with control group,protein levels of Bcl-2 and phosphorylated ERK were increased in 100,200,400 mg/L LBP intervene group （F =65.26,P ＜0.01 ; F =14.85,P ＜0.01）,and decreased in 400 mg/L LBP intervene group.The difference of levels of Bcl-2 and phosphorylated ERK were statistically significant between 200 mg/L LBP intervene group and 400 mg/L LBP intervene group （all P ＜ 0.01）.Conclusions Lower concentration LBP could promote the proliferation and inhibit the apoptosis of MIN6 cells,while the effects of higher concentration LBP were on the contrary.The mechanisms were associated with ERK signaling pathway.
出处
《国际内分泌代谢杂志》
北大核心
2013年第5期298-301,共4页
International Journal of Endocrinology and Metabolism
基金
国家自然科学基金资助项目（81170769）
关键词
枸杞多糖
MIN6细胞
细胞增殖
细胞凋亡
Lycium barbarum polysaccharide
MIN6 cells
Cells viability
Apoptosis
