摘要
目的 探讨慢性脑血流灌注不足认知功能障碍和环胞素A治疗作用。方法 持久性双侧颈总动脉结扎所致慢性脑血流灌注不足的大鼠模型 ;用电脑控制穿梭箱记录大鼠学习记忆能力 ;用免疫组化研究脑内血源性白细胞和T细胞浸润的规律、分布和可能作用及免疫抑制剂环胞素A(CsA)的治疗效果。结果 慢性脑血流灌注不足 2～ 4个月大鼠的学习记忆能力明显下降 ;缺血 1个月后皮层、海马和白质有白细胞和T细胞的浸润 ,2～ 4月浸润的白细胞和T细胞减少 ;经CsA治疗者细胞浸润和学习记忆能力明显好转。结论 CsA可以抑制慢性脑血流灌注不足后脑内白细胞和T细胞的入侵 ,减轻脑损害 。
Objective To explore the cognitive function disturbance and efficiency of cyclosporin A (CsA) in rats after chronic cerebral hypoperfusion (CCH). Methods The CCH model was established in Wistar rats with bilateral ligation of common carotid artery. The cognitive function of rats was measured by a computerized shuttle training case. The infiltration of leukocytes and T cells in the brain was observed with immunohistochemical methods. The efficiency of CsA treatment was studied. Results The cognitive function was decreased obviously in rats with 2 to 4 months of CCH. There were leukocytes and T cells infiltrating into the cortex, the hippocampus and the white matter of the brain after 1 month of CCH, but the amount of the cells was reduced within 2 to 4 months. CsA could alleviate the cell infiltration and the rat cognitive function. Conclusion CsA can inhibit the infiltration of leukocytes and T cells in the brain, attenuate brain injury and improve the cognitive function of rats after CCH.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第11期1042-1045,共4页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 !(39770 2 73)
