摘要
目的 :探讨扩张型心肌病 (DCM)的体液免疫学发病机制。方法 :借助免疫转印技术及流式细胞仪技术同步分析了 33例 DCM患者和 2 8例正常人的血清中抗心肌线粒体 ADP/ATP载体自身抗体和 Th细胞亚群。结果 :DCM组 Th2 细胞在外周血淋巴细胞中占 (0 .5 3± 0 .2 3) % ,对照组 Th2 细胞占 (0 .2 2± 0 .18) % ,两组比较差异有显著性 (P <0 .0 1) ;DCM组抗心肌线粒体 ADP/ATP载体抗体的检出率 (6 0 .6 1% )明显高于对照组(2 1.43% ) (P <0 .0 1) ;DCM抗心肌线粒体 ADP/ATP载体自身抗体 ,阳性组中 Th2 细胞在外周血淋巴细胞中占 (0 .6 7± 0 .31) % ,阴性组 Th2 细胞占 (0 .2 8± 0 .14) % ,两组比较差异有显著性 (P<0 .0 1)。结论 :DCM中 Th2细胞与抗心肌线粒体 ADP/ATP载体自身抗体的产生有关 ,而且 Th2 细胞和抗心肌线粒体 ADP/ATP载体自身抗体参与了 DCM的病理过程。体液免疫反应的异常在 DCM的发病机制中起着十分重要的作用。
Objective:To investigate the pathogenesis of humoral immunology of dilated cardiomyopathy (DCM).Method:The autoantibody against myocardial mitochondria ADP/ATP carrier and the Th cell subsets was examined by using the immunoblot analysis and the flow cytometry in 33 patients with DCM and 28 normal control subjects.Result:The percentage of Th 2 cells detected in patients with DCM〔( 0.53 ± 0.23 )%〕were significantly higher than those in controls〔( 0.22 ± 0.18 )%〕,P< 0.01 .The percentage of the autoantibody against myocardial mitochondria ADP/ATP carrier in patients with DCM ( 60.61 %)were significantly higher than those in controls ( 21.43 %),P< 0.01 .The percentage of Th 2 cells in DCM patients with autoantibody against myocardial mitochondria ADP/ATP carrier positive ( 0.67 ± 0.31 )% were significantly higher than those with negative( 0.28 ± 0.14 )%, P< 0.01 .Conclusion:Th 2 cells is related to the production of autoantibody against myocardial mitochondria ADP/ATP carrier in DCM,and Th 2 cells and the autoantibody against myocardial mitochondria ADP/ATP carrier takes part in the pathogenesis of DCM.Our results further verify that abnormality of humoral immunity plays an important role in the pathogenesis of DCM.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2000年第11期503-505,共3页
Journal of Clinical Cardiology
基金
卫生部资助!课题 (No.96 - 2 - 10 5 )
