摘要
目的 观察脑组织中脂质过氧化物含量和脂肪酸组成的变化。方法 将 36只Wistar大鼠分成 3组 ,分别饲以相同饲料、不同浓度含氟水 ,5个月后处死。用铁离子诱导氟中毒大鼠脑组织脂质过氧化反应 ,用高效液相色谱方法测定脑组织中丙二醛含量 ,用氧电极方法测定脂质过氧化过程中的氧耗量 ,用气相色谱方法测定脑组织中各类脂肪酸组成。结果 用自由基诱导剂激发脂质过氧化后 ,与对照组动物相比 ,氟中毒动物脑组织中丙二醛明显增多 ,氧耗量增加 ,多不饱和脂肪酸构成减少 ,饱和脂肪酸组成增加。
Objective To study changes in content of lipid peroxide and compositon of fatty acids in the brain of rats afflicated with chronic fluorosis after treatment with free radical inducer (ferric ion). Methods Thirty six Wistar rats were divided into three groups, fed with similar fodder and varied concentrations of fluoride in drinking water, and were killed five months after treatment. Lipid peroxidation was induced by ferric ions. Malondialdehyde content in brain was analysed by high performance liquid chromatography; oxygen consumption was determined with an oxygen electrode and fatty acid composition was measured by gas chromatography in brain tissues of the rats. Results In the brain tissues, content of malondialdehyde and oxygen consumption increased, composition of polyunsaturated fatty acids decreased and that of saturated fatty acids decreased after treatment with free radical inducer in the treated group, as compared with those in control group. Conclusion Over uptake of fluoride for a long term could cause potantial increase in the level of oxidative stress in the brain tissue.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2000年第6期330-332,共3页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助(9839760029)
