摘要
目的观察丙泊酚对内毒素诱发的急性肺损伤的保护作用及与肺上皮细胞核内Nrf2表达的关系。方法腹腔注射内毒素建立小鼠急性肺损伤模型,实验动物分为对照组、内毒素组、内毒素+丙泊酚组、内毒素+脂肪乳剂组和丙泊酚组,分别检测小鼠生存率、肺湿重/干重比、支气管肺泡灌洗液蛋白浓度和白细胞计数、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量、肺上皮细胞核内Nrf2水平。结果腹腔注射内毒素导致小鼠生存率降低,肺湿重/干重比、支气管肺泡灌洗液蛋白浓度和白细胞计数增加,SOD活性降低和MDA含量增加,丙泊酚能明显提高小鼠肺上皮细胞核内Nrf2表达。结论丙泊酚对内毒素诱发的急性肺损伤具有保护作用,这可能与丙泊酚激活肺上皮细胞核内Nrf2表达有关。
Aim To investigate the correlation of pro- tective effect of propofol on lipopolysaccharide-induced （LPS-induced） acute lung injury with nuclear Nrf2 ex- pression of lung epithelial cell. Methods The model of acute lung injury in mice was established by intrap- eritoneal injection （ip） LPS, the mice were randomly divided into Control group, LPS group, LPS ＋ Propgroup, LPS ＋ Inp group and Prop group, mice survival rate, lung wet/dry weight ratio, total protein concen- tration and WBC count in （bronchoalveolar lavage flu- id） BALF, SOD activity and MDA content, and nucle- ar Nrf2 expression of lung epithelial cell were deter- mined. Results LPS injection led to a decrease of mice survival rate , increases of lung wet/dry ratio, total protein concentration and WBC count in BALF, a depression of SOD activity and an increase of MDA content. Propofol significantly enhanced nuclear Nrf2 expression of lung epithelial cell. Conclusion Propo- fol has protective effects on LPS-induced acute lung in-jury, which may be relevant to activation of nuclear Nrf2 expression by propofol.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2013年第9期1286-1290,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 30872432
30471657
39970715)
关键词
丙泊酚
内毒素
急性肺损伤
超氧化物歧化酶
丙二醛
激活
Nrf2表达
propofol
lipopolysaccharide （ LPS ）
a- cute lung injury
superoxide dismutase
malondialde- hyde
activation
Nrf2 expression
