摘要
以抗病材料Ryau96172I和感病材料Rutagus以及所需鉴定的番茄品种为试验材料,根据Mi-1.2和Mi-1.1设计引物,利用改良SDS法提取番茄基因组DNA,用锚定引物扩增多态性DNA分子标记法标记不同番茄品种。同时利用爪哇根结线虫虫卵以5 000粒/株,接种苗龄为25 d,室内人工接种不同番茄品种。接种结果得到抗病材料2份、中抗材料2份、高感材料5份。本试验对APAPD分子标记反应体系的退火温度进行了优化,适宜退火温度为38℃。应用该体系筛选得到特异性引物1条,命名为SF30,特异片段长度为408 bp。综合以上两者结果表明,APAPD分子标记的结果与接种爪哇根结线虫鉴别番茄品种抗性结果相吻合。
The test materials were the resistant material Ryau96172I, susceptible material Rutagus and varieties of required identification tomatoes. According to the Mi-1. 2 and Mi-1. 1 gene, we de-signed primers, the genomic DNA of tomato was extracted by improved SDS method, using anchored primer amplification polymorphism DNA molecular markers. In addition, we inoculated tomato varie ties when the seedling age was 25 d, using Meloidogyne javanica eggs （5 000 per plant） , to distinguish varieties of tomatoes of root-knot nematode disease resistance. The results obtained 2 resistance materi- als, 2 medium resistance materials and 5 highly susceptibility materials. In this study, we optimized the annealing temperature of APAPD molecular markers reaction system, and the best annealing tem- perature was 38℃. Screening of different primers was obtained a specific primer by the best system, named SF30, and the specific fragment length was 408 bp. The results of M. javanica inoculation and APAPD molecular markers were the same.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第5期631-636,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省科技计划项目(2010BB014)
关键词
番茄
根结线虫病
锚定引物扩增多态性DNA
分子鉴别
tomato
root-knot nematode
anchored primer amplification polymorphism DNA（APAPD）
molecular identification
