摘要
目的比较梅毒螺旋体抗体颗粒凝集试验（TPPA）、梅毒酶联免疫吸附试验（TP—ELISA）、快速血浆反应素环状卡片实验（RPR）、胶体金快速检测试验（SYP）4种梅毒检测方法，选择一种适合医院梅毒检测的模式。方法（1）分别用以上四种方法检测滨州医学院附属医院门诊2012年10月。2013年5月间确诊为梅毒的患者135例，其中一期梅毒46例，二期梅毒39例，三期梅毒5例，潜伏梅毒45例。（2）2012年10月～2013年5月间我院住院患者术前或输血前抽取血样32066份，先用TP—ELISA法检测，发现阳性266例，再用TPPA、RPR、SYP法分别检测。结果（1）第一组试验结果：TPPA、ELISA、RPR、SYP四种梅毒检测方法的敏感度分别是98．78％、98．78％、78．52％、91．11％。TPPA、TP—ELISA间差异无统计学意义，TPPA、ELISA、RPR、SYP四种梅毒检测方法的前两种与后两种间以及后两种互相之间差异有统计学意义。（2）第二组试验结果：266例TP—ELISA阳性标本分别用TPPA、RPR、SYP法检测，阳性率分别是85％、69％、79％。TPPA、RPR、SYP三种试验彼此间差异有统计学意义。结论TPPA法适用于其他方法检测阳性后再进行确诊试验，或者工作量较小的医疗机构；RPR适合基层医院的梅毒筛查和治疗疗效观察；TP—ELISA法适合工作量较大的医疗机构进行初筛检验；SYP法适用于基层医院或急症检测，但检测后应用其它方法进行复检。
Objective To compare four kinds of syphilis testing methods of antibody of treponema pallidum particle agglutination （TPPA）, treponema tllidum enzyme-linked immunosorbent assay （TP-ELISA）, rapid plasma reagin circle card test（RPR）, colloidal gold rapid testing （SYP）, and choose a model for clinical syphilis testing. Methods （1） Four methods were used to detect more than 135 cases are known to out-of Binzhou Medical College Hospital from October 2012 to May 2013 among patients diagnosed with syphilis. Among those patients, in which 46 cases of primary syphilis, 39 cases of secondary syphilis, tertiary syphilis in 5 cases, 45 cases of latent syphilis. （2） From 2012 October to May 2013 patients in our hospital before surgery or blood transfusion before the blood samples were drawn 32066 copies, first with the TP-ELISA assay and found positive for 266 cases, and then TPPA, RPR, SYP were detected. Results （1） The first set of test results： TPPA, ELISA, RPR, SYP four species sensitivity of syphilis testing methods were 98.78%, 98.78%, 78.52%, 91.11%. TPPA, TP-ELISA had no significant difference, TPPA, ELISA, RPR, SYP 4 kinds of syphilis testing methods between the first two and last two and last two has significant differences between each other. （2）The second group of test results： 266 cases were TP-ELISA positive samples with TPPA, RPR, SYP assay, the positive rate was 85%, 69%, 79%. TPPA, RPR, SYP three tests were significantly different between each other. Conclusion TPPA method applies to be the confirmatory test after other methods, or a smaller workload of medical institutions. RPR for primary syphilis screening and therapeutic evaluation of primary hospital patients. TP- ELISA method is suitable for the larger medical institutions work screening test. SYP method is suitable for primary care hospital or emergency testing, but the test should be re-examination by the application of other methods.
出处
《中国医药科学》
2013年第18期19-21,共3页
China Medicine And Pharmacy
