摘要
我们在长期的超薄切片实践中发现,在刀根小于1mm的条件下,玻璃6刀口的最佳切割位置与传统概念不相符合,甚至相反,即右侧刀口(通常认为不能用来切片)不但不是切片“禁区”,而且比左侧刀口(传统认为是最佳刀口)锋利、耐用。为此,我们利用光学显微镜(LM)、扫描电镜(SEM)及透射电镜(TEM)对刀口的不同部位及其切片进行了观察研究,为如何正确使用和改进玻璃刀口提供了有价值的资料。
In a long period of ultramicrosomy practice, we found that the site of perfect cutting edge of a glass knife is
contrary to the traditional concept if the height of the knife heel is controled whthin 1mm. The right cutting edgewhich is considered to be the saw teeth side and can not be used for ultra-thin sectioning is not a restricted side.It is even sharper and more durable than the left which is traditionally believed to be the perfect cutting edge.The lightmicroscope (LM), the scanning electron microscope (SEM) and the transmission electron micro-scope (TEM) have been used to observe the different sides of cutting edge and the semi and ultra thin sections cut by the different sides. Some results have been obtained which provide useful information for proper use and improvement of glass-knife cutting edge.
出处
《电子显微学报》
CAS
CSCD
1991年第3期307-310,共4页
Journal of Chinese Electron Microscopy Society
