摘要
目的 :用基因芯片研究 PMA激活的血管内皮细胞的早期反应基因 (imm ediate early response gene,ERG)。方法 :以包含 40 96条人类基因的 DNA芯片检测血管内皮细胞受代谢增强剂 PMA(phorbol myristate acetate)激活后早期的基因表达谱 ,并从中筛查出 ERG。结果 :血管内皮细胞受 PMA作用 6 h后 ,17条基因上调 ,11条下调。数据处理聚类分析表明 17条上调基因中多数 (13/ 17)属蛋白质磷酸酶和转录调控因子基因 ,而下调基因中多数 (9/ 11)为细胞分裂相关基因。结论 :证明PMA激活的人血管内皮细胞早期反应基因主要是转录调控因子和蛋白质磷酸酶基因。
Objective: [WT5”,6BZ]To study endothelial early responses to PMA by genomic scale gene expression analysis. Methods: The response of endothelial cells to PMA was explored with a complementary DNA microarray representing 4 096 different human genes. Results: Genes was clustered into groups on the basis of their temporal patterns of expression in this study. The results of mRNA profiles analysis indicated that 17 of genes were upregulated while 11 were downregulated. Interestingly, most of the genes (13/17) upregulated by PMA were involved in signal transduction and transcription, while 11 of genes down regulated were mainly genes related to cell proliferation (7/11). suggesting that endothelials play a larger and richer role in this complex multicellular response than previously thoaght. Conclusion: The immediate early genes (ERGs) in human blood vuscular endothelial cell treated with PMA are dominated by transcription factors as well as protein phosphatases. [
出处
《第二军医大学学报》
CSCD
北大核心
2000年第9期815-818,I002,共4页
Academic Journal of Second Military Medical University
基金
上海市现代生物与新药产业基金!资助项目 ( 9843 1912 1)
关键词
微矩阵基因芯片
血管内皮细胞
早期反应基因
PMA
gene microarray
endothelial cell
early response genes
transcription factor
protein phosphatase
