摘要
目的探讨p38MAPK信号转导通路对变应性鼻炎(allergic rhinitis,AR)中细胞因子白细胞介素6(IL-6)和肿瘤坏死因子(TNF-α)表达的调控作用及p38MAPK与IL-6、TNF-α之间的相关性。方法变应性鼻炎(AR组)及正常者(正常组)各20例,分别从每位受试者的外周血中分离出单核细胞并分成3组培养。第1组为空白对照,第2组加入P38MAPK激动剂12-肉豆蔻酰-13-乙酸佛波酯(phorbol12-myristate13-acetate,PMA),第3组同时加入PMA和P38MAPK特异性抑制剂SB203580,将单核细胞进行培养,westernblot法检测核蛋白P38MAPK的表达,RT-PCR法检测IL-6和TNF-α的表达,ELISA法检测上清液中的IL-6和TNF-α蛋白含量。结果加入PMA培养的AR组T单核细胞P38MAPK蛋白表达、IL-6和TNF-αmRNA的表达及培养上清液中的IL-6和TNF-α蛋白表达与空白对照组比较均明显增加(均p<0.01);且与加入PMA培养的正常T淋巴细胞组比较上述指标也均明显增加(均p<0.01);而同时加入PMA和SB203580培养的AR组单核巴细胞的上述指标与加入PMA培养的AR组比较明显降低,但与AR空白对照组比较仍有增加(均p<0.01)。单核细胞P38MAPK蛋白表达与IL-6和TNF-α的mRNA表达均存在正相关(均p<0.01),与培养上清液中的IL-6和TNF-α蛋白含量也均存在正相关(均p<0.01)。结论p38MAPK信号转导途径参与调节AR的单核细胞活化及IL-6、TNF-α的基因表达。
Objective To explore the role of p38 mitogen -activated protein kinase（p38MAPK） in the signal pathway regulating the expression of interleukin 6 （ IL -6） TNF - α, by T lymphocyte in allergic rhinitis. Methods 20 acute episode allergic rhinitis patients and 20 normal control persons participated in the study, monoeyte isolated and purified from blood of each person were divided into 3 groups ： cells of 1st group were control group, cells of 2nd group were stimulated with phorbol 12 -myristate 13 -acetate （PMA）, cells of 3rd group were stimulated with PMA and SB203580. The expression of p38MAPK was observed by Western blot, the expression of IL - 6 and TNF - α mRNA were observed by RT- PCR, IL- 6 and TNF -α protein in supematants were measured by ELISA. Results The expression of nuclear p38MAPK protein, IL - 6 and TNF - α mRNA, IL - 6 and TNF - α protein in supematants of allergic rhinitis monoeyte stimulated with PMA were significantly higher than those of allergic rhinitis monocyte stimulated without PMA（p 〈0. 01 ,respectively） and those of normal control monooyte stimulated with PMA （p 〈 0. 01 ,respectively）,and were significant reduced by SB203580 （p 〈0. 01 ,respectively）. Those of index in allergic rhinitis monocyte stimulated with PMA and SB203580 were higher than those of allergic rhinitis monooeyte stimulated without PMA （p 〈 0.01, respectively）. There were good positive eorrelation between the expression of p38 MAPK protein, IL -6 and TNF - α mRNA （p 〈0. 01, respectively） ,and also good positive correlation between the p38MAPK protein and IL -6 and TNF - α protein in supernatants （p 〈 0.01, respectively）. Conclusion The active p38MAPK of allergic rhinitis activated monocyte and up - regulated expression of IL - 6 and TNF - α. The activation of p38MAPK signal pathway of monooyte participated in morbidity of allergic rhinitis.
出处
《现代医院》
2009年第8期22-24,共3页
Modern Hospitals
