摘要
双向PCR扩增特异等位基因 (Bi PASA)是一项建立在PCR基础之上的分子生物学新技术。它通过 2对引物的一次PCR反应可以迅速、有效地检测出基因组中单个碱基的突变 ,从而识别个体基因型的差异。现以猪氟烷基因即兰尼定受体 (Ryanodinereceptor 1 )基因的多态性检测为例 ,介绍该技术的基本原理。
Bidirectional PCR amplification of specific alleles(Bi PASA) is a simple method to distinguish single base changes and to genotype homozygotes and heterozygotes in a single PCR reaction.Two alleles are amplified simultaneously and in opposite directions relying on allele specific PCR.The technique requires two pairs of novel primers(two outer primers and two allele specific inner primers) and appropriate cycling conditions.The overview summarized the development and general protocols with an example of RYR1 gene.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2000年第2期69-71,共3页
Journal of Nanjing Agricultural University
关键词
Bi-PASA技术
RYR1基因多态性
杂合子
纯合子
bidirectional PCR amplification of specific alleles
RYR1 gene
polymorphism
inspection technique
