摘要
本研究以PCR RFLPs检测方法 ,分析了RYR1基因在山东省引进猪种及其杂交后代各群体中的分布。在纯种猪中 ,皮特兰猪的n基因的基因频率最高 (10 0 % ) ,其次为长白猪 (11 4 4% ) ,再次为约克夏猪 (6 68% ) ,杜洛克和汉普夏猪为 0 ;在杂种群体中 ,杜洛克×皮特兰猪最高 (5 0 % ) ,长白×约克夏猪为 10 62 % ,约克夏×长白猪为 7 86% ,约克夏×长白与长白×约克夏杂交所得的F2代为 1 31% ,杜洛克×汉普夏和汉普夏×杜洛克猪为 0。利用最小二乘模型分析了RYR1基因对生产性能的遗传效应 ,结果表明 :RYR1基因对 2 0日龄体重和 60日龄体重的影响不显著(P >0 0 5 ) ,但极显著提高日增重 (P <0 0 1)。RYR1基因可极显著地提高猪的胴体瘦肉率 ,降低猪肉的肉色评分 (P <0 0 1)。NN、Nn和nn 3种基因型对瘦肉率和肉色评分的最小二乘均值分别为 64 0 0 %、66 87%、67 87%和 2 73、2 5 7、2 0 2。在繁殖性状方面 ,RYR1基因对仔猪出生重影响不显著 (P >0 0 5 ) ,但显著降低繁殖母猪的产活仔数 (P <0 0 5 ) ,NN、Nn和nn 3种基因型对产活仔数的最小二乘均值分别为 11 16、10 0 0、8 2 6头。
By PCR RFLPs the present paper analysed the gene frequency of the RYR1 gene in purebred and crossbreeding swine populations in Shandong province.In the purebred populations,gene frequency of n gene in Pietrain was the highest(100%),in Landrace-11 44% and Yorkshire-6 68%.No n gene was detected in Duroc or Hampshire pigs.In the crossbreeding swine populations,the frequency of the n gene in Duroc ×Pietrain was 50%,Landrace×Yorkshire -10 62%, Yorkshire×Landrace-7 86%.The F 2 offspring population of Yorkshire×Landrace crossed with Landrace×Yorkshire-1 31%,Duroc×Hampshire-0 and Hampshire×Duroc-0.By least square statistical analysis little effect of RYR1 gene on the body weight at 20 days and at 60 days (P>0 05) was proved.But the RYR1 n gene increased the daily gain significantly (P<0 05),it increase the rate of lean carcass and decrease the value of meat color (P<0 01) as well.The values of least square mean of the NN,Nn,nn genotypes on the rate of lean carcass and meat color were 64 00%,66 87%,67 87% and 2 73,2 57,2 02,respectively.In terms of reproduction traits,the RYR1 gene had little effect on the piglet's body weight at birth,but significantly decrease the dam's litter size (P<0 05).The effect of NN,Nn,nn genotypes on litter size were 11 16,10 00 and 8 26,respectively.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2001年第6期481-486,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山东省科委良种产业化项目资助
关键词
猪
RYR1基因
生产性能
遗传效应
Pig
RYR1 gene
PCR RFLPs
Performance traits
Least square
