摘要
目的 探索放射联合肿瘤引流区淋巴结淋巴细胞 (tumordraininglymphnodelymphocyte,DNL)对肿瘤细胞的作用。方法 取首次治疗病理证实的口腔癌患者颈清扫淋巴结 ,经常规消化、分离 ,加白细胞介素 2 (interleukin 2 ,IL 2 )培养 ,获取激活的淋巴细胞作为效应细胞 ,以效靶比 (E∶T) 2 5∶1进行集落形成试验 ,观察对人涎腺腺样囊性癌肺高转移细胞株 (adenoidcysticcarcinoma ,ACC M)的抗瘤效应 ,并分别以不同剂量60 钴γ射线对ACC M照射后 ,随即加入DNL细胞 ,观察放射结合DNL对ACC M的抗瘤效应。结果 ACC M为放射中度敏感细胞。根据线性———二次数学模型 (LQ模型 ) ,其α、β值分别为 0 342 0 ,0 0 783。DNL对ACC M的抑瘤率为 49 0 6 %。放射联合DNL作用时 ,α值提高到 0 76 88;Dq、S2 分别从 1 5 90 1、0 44 81降至 0 5 995、0 113 5 ,这 3个参数与放射组相比 ,差异均有显著性 (P <0 0 5 )。在高剂量区 ,与放射组比 ,放射联合淋巴细胞组的D0 0 1和 6Gy、8Gy剂量点的细胞存活率均明显下降 ,P <0 0 1。结论 体外来源于不同病理类型口腔癌的IL 2激活的DNL对ACC M有明显的抗瘤效应 。
Objective To find whether there is any synergistic effect of radiation combined with interleukin 2(IL 2) activated tumor draining lymph nodes lymphocyts (DNL) from oral carcinoma patients on high lung metastatic salivary adenoid cystic carcinoma cell line(ACC M) Methods Colony forming test was used to investigate antitumor effect and analyzed using linear quadratic(LQ) equation and single hit multi targets equation Results The ratio of effect to targets was 25∶1 The cytotoxiclty of DNL was 49 06% Radiation combined with DNL showed higher antitumor activity compared with radiation alone,α value, Dq and S 2 were 0 768 8 and 0 342 0;1 590 1 and 0 599 5; 0 448 1 and 0 113 5 respectively( P <0 05) Conclusions It indicates that in initial region of survival curve, DNL significantly increased sublethal damage on ACC M
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2000年第4期245-247,共3页
Chinese Journal of Stomatology
