摘要
作者采用￣５１Ｃｒ４ｈ释放试验和裸鼠移植瘤模型，从颈淋巴清除术后标本中制取肿瘤引流区淋巴结细胞（ＤＮＬ），经体外激活后与自身ＬＡＫ细胞比较对人舌鳞癌细胞系Ｔｃａ８１１３细胞体外、体内的杀伤活性，结果显示：Ｅ：Ｔ为２５：１时，ＤＮＬ组体外杀伤率为６５％，ＬＡＫ组为２８％，按Ｗｉｎｎ氏中和法分别将ＤＮＬ，ＬＡＫ细胞同Ｔｃａ接种于裸鼠皮下，结果ＤＮＬ组抑瘤率为７４％，ＬＡＫ组为４２％，经统计学分析两组间存在显著性差异（Ｐ＜０．０５），提示ＤＮＬ细胞杀瘤活性明显强于ＬＡＫ细胞，且来源容易，与传统的ＬＡＫ细胞比较在癌过继免疫治疗中有较高的实用价值。
AbstractThe in vitro and in vivo antitumor activrty be-tween DNL and autologous LAK cells was compared.The resuhs showed that DNL cells activated in vitroby rIL-2 exhibited higher cytotoxicity to Tca 8113cells than the same patient's LAK cells (75% and36% , respectively). According to Winn's method,the inhibition rates of subcutaneously transplantedTca cells in mice were 74% and 42% respectively.There was significantly difference in the antitumor ac-tivity in vitro and in vivo between two groups (P<0. 003 , indicating that DNL cells which are moreavailabe are more practicable for adoptive im-munotherapy.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1994年第6期336-338,共3页
Chinese Journal of Stomatology
基金
上海市卫生局青年科研基金
