摘要
目的:观察大肠腺癌,腺瘤恶变区及非恶变区细胞凋亡及其调控基因p53的表达状态,探讨它们在大肠上皮恶性转化进程中的作用及二者的关系。方法:利用DNA缺口末端标记技术,p53蛋白免疫组化染色及双重染色技术,原位观察27例大肠腺癌及21例腺瘤恶变标本中凋亡细胞和p53阳性表达细胞的密度与分布,以8例非肿瘤大肠粘膜作为对照。结果:腺瘤非恶变区凋亡细胞密度分别高于腺癌(P<0.01)、腺瘤恶变区(P<0.01)及非肿瘤粘膜(P<0.01)。腺癌及腺瘤恶变区p53蛋白阳性细胞密度均高于腺瘤非恶变区(P<0.01)及非肿瘤粘膜(P<0.01)。双染原位观察p53蛋白与凋亡细胞的分布发现:p53阳性区凋亡细胞有明显减少趋势,而p53阴性区凋亡细胞分布则有增加趋势。结论:p53蛋白突变在大肠癌发展中具重要的促恶变形成作用,其作用可能是通过抑制细胞凋亡而实现的。
To investigate the relationship between p53 and apoptosis in different stages of malignant transformation of large intestine epithelia. Methods:Using DNA nick end labeling technique for apoptotic cells, immunohistochemical staining for p53 protein and double staining for both,the density and distribution of the apoptotic cells and positive expression of p53 protein were observed in situ in 21 cases of malignantly transformed adenomas and 27 cases of adenocarcinomas of the large intestine. Fifteen cases of non-tumor mucosas were used as controls. Results:The density of the apoptotic cells in benign areas of adenomas was significantly higher than that in non-tumor mucosas,malignant areas of adenomas or adenocarcinomas respectively(all P < 0.01). The densities of p53 protein positive cells in malignant areas of adenomas and adenocarcinomas were significantly higher than those in benign areas of adenomas and non-tumor mucosas respectively (all P< 0.01). The distributions of p53 protein and apoptotic cells observed through double staining showed a tendency to decrease of apoptosis in p53 protein positive areas, while showing a tendency to increase in p53 negative areas. Conclusion: Probably through decreasing in apoptosis,p53 mutations play a critical part in the formation and progression of carcinomas of large intestine.
出处
《天津医药》
CAS
2000年第11期665-667,共3页
Tianjin Medical Journal
基金
天津市自然科学基金
