摘要
【目的】探讨癌胚抗原重组痘苗病毒 (CEA rV)对癌胚抗原 (CEA)阳性肿瘤的抑瘤机制。【方法】使用我室构建的CEA rV腹腔接种C5 7BL/ 6小鼠 3次 ,6周后 ,取其腹腔巨噬细胞 (MΦs)转输给携带CEA阳性肿瘤的受体小鼠 ,同时检测供体MΦs及受体脾细胞的体外细胞毒活性。【结果】供体MΦs体外细胞毒活性无明显增加 ,但体内却能明显抑制CEA阳性肿瘤的生长 ,受体脾细胞对同一靶细胞的杀伤活性亦明显增强。【结论】经CEA rV接种处理的小鼠MΦs可能通过其抗原提呈作用 ,激活T淋巴细胞 ,进而通过T细胞的特异性抑瘤活性 ,杀伤CEA阳性肿瘤细胞。
Objective To explore antitumor mechanism of carcinoembryonic antigen-recombinant vaccinia virus(CEA-rV). Methods C57BL/6 mice were immunized three times with rV-CEA. Six weeks later, the macrophages(M Φs) from CEA-rV-immunized donors were adoptively transfered in CEA +-HePa-bearing recipients,meanwhile, the antitumor effects of the donor M Φs and of the recipients splenocytes in vitro were detected. Results The antitumor effect in vitro of the M Φs from CEA-rV-immunized donors was the same as those from contrals statistically. However, the M Φs expressed strong antitumor activity in CEA positive tumor-bearing recipients,the antitumor effect in vitro of the recipients splenocytes was statistically increased. Conclusion It was demonstrated that antitumor activity induced by CEA-rV was essentially mediated by antigen pressent cells such as the M Φs which activated immune T cells by CEA-specific immune response to kill tumor cells.
出处
《中山医科大学学报》
CSCD
2000年第6期429-431,480,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广州市科委基金资助项目(JB02-98-5-018-01)
广东省卫生厅科研基金资助项目(A1998127)
