摘要
探讨间接法原位 PCR在弓形虫感染病原学检测中的应用。以 RH株弓形虫速殖子感染小鼠后第 2、4、6天处死 ,取肝脏制备石蜡标本及冰冻切片标本 ,以间接法原位 PCR扩增并检测石蜡标本中的弓形虫 DN A,与原位杂交方法检测冰冻切片标本的结果比较。以间接原位 PCR方法检测 18例标本均得到阳性信号 ,病原体检出率为 10 0 % ,优于原位杂交法 ( 9/18)。间接法原位
The possible application of the indirect in situ PCR technique or detection of Toxoplasma infection in liver sections of experimentally infected mice was studied. Mice inoculated with Toxoplasma gondii tachyzoites (RH strain) were sacrificed 2,4,6 days after infection Paraffin and frozen sections of the liver were prepared. The indirect in situ PCR was employed to amplify and detect the specific parasite DNA with the paraffin sections. The in situ hybridization technique using frozen sections was parallelly compared. Specific T.Gondii DNA was demonstrated in all the 18 paraffin sections using the indirect in situ PCR; while only half of the frozen (9/18) showed positive by the in situ hybridization technique. The indirect in situ PCR technique may provide a feasible new tool pathological detection of Toxoplasma DNA using normal paraffin sections.
出处
《寄生虫与医学昆虫学报》
CAS
2000年第2期65-69,共5页
Acta Parasitologica et Medica Entomologica Sinica
基金
北京市青年科技骨干培养基金
关键词
弓形虫
病理检测
间接原位PCR
诊断
Toxoplasma gondii Pathological detection Indirect in situ PCR
