摘要
籽粒大小是影响玉米产量的关键因素,百粒重是衡量籽粒大小的一个指标。本研究基于59份玉米染色体单片段代换系(SSSL)纯合体,对玉米百粒重性状进行2年6个试验环境的表型鉴定,利用t测验和重叠群作图的方法对SSSL内代换片段上的百粒重效应进行了QTL分析。共检测出20个百粒重QTL,分布在玉米的8条染色体上,其中14个(70.0%)在2个以上试验环境中被重复检出,4个(20.0%)在4个以上试验环境中被重复检出,在全部6个试验环境中重复检出且基因加性效应值较大的玉米百粒重QTL是位于玉米第5染色体Bin5.05区SSR分子标记bnlg278和umc1680附近的q100kw-5-3。研究结果为玉米籽粒大小性状相关基因的进一步精细定位和克隆奠定了基础。
Kernel size is a key component of maize yield. Fifty-nine homozygous chromosome single segment substitution lines （SSSL） were employed to identify the QTL of 100-kernel weight of maize under six environments using t-test and overlap map- ping method. Twenty QTLs of 100-kernel weight were identified on eight chromosomes of maize, of which fourteen QTLs （70.0%） were found repeatedly in more than two environments, four QTLs （20.0%） were found repeatedly in more than four en- vironments, the major QTL qlOOkw-5-3, which was detected repeatedly under six environments, was located near the SSR mark- ers bnlg278 and umc1680 at Bin 5.05. These results provide a good foundation for further fine mapping and cloning of major genes related to the maize kernel size.
出处
《作物学报》
CAS
CSCD
北大核心
2013年第9期1562-1568,共7页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30871580)
国家转基因生物新品种培育科技重大专项(2009ZX08009-111B)资助
