摘要
目的探讨抗体筛查细胞抗原涵盖范围在保障电子配血技术安全应用中的意义。方法分别对供受血者进行ABO/RhD血型检测以及抗体筛查。患者二次血型鉴定结果一致,抗体筛查结果阴性,按电子配血规则进行电子配血,同时采用凝聚胺法对供受血者进行传统血清学交叉配血平行试验,分析抗体筛查细胞抗原涵盖范围与不规则抗体漏检情况以及对电子配血技术安全应用的影响。结果 22790份患者血样,抗体筛查阳性164例,阳性率0.72%。15740份供血者血样,抗体筛查阳性11例,占0.07%。22540份血样均符合电子配血规则,由计算机实施电子配血未发现ABO/RhD血型不相容。用凝聚胺法进行传统血清学交叉配血平行检测,其中22534份血样配血相容,6份血样凝聚胺法交叉配血主侧不相容,未发现次侧不相容者。6份血样用谱细胞进行抗体特异性鉴定,结果全为MNS系统抗-Mur,漏检率为0.027%。结论目前临床所用抗体筛查细胞仅为血清学交叉配血而设计,抗体漏检率相对较高,不能充分保障电子配血技术的安全性。建议尽快制定电子配血技术标准,保障其在临床的推广应用。
Objective To study the significance of antibody screening cell antigen range in the electronic matching technology security application. Methods ABO/RhD of blood donor and blood recipient were detect with blood type and antibody. The blood matching were used by electronic matching technology and polybrene crossmateh. Results Of 22, 790 blood samples of patients, there were 164 cases with positive antibody screening （0. 72%）. Of 15740 blood donor blood samples, there were 11 cases with positive antibody screening （0.07~）. Of 22,540 blood samples in line with the rules of the electronic distribution of blood, the implementation of electronic matching of blood ABO / RhD blood group incompatibility was not found by the computer. Of 22534 blood samples with blood compatible, 6 blood samples poly- brene crossmatch was incompatible with the main side and found no secondary side incompatible. 6 blood samples spec- trum cell antibody specific identification of all the MNS system anti-Mur, missed rate of 0. 027%. Conclusion Clinical antibody screening cells only serological crossmateh, whose antibody missing rate is relatively high, which cannot fully protect the security of the technology of the electronic matching of blood.
出处
《西部医学》
2013年第9期1401-1403,共3页
Medical Journal of West China
基金
四川省卫生厅科研课题(120023)
关键词
抗体筛查
不规则抗体
抗原谱
电子配血
输血安全
Antibody screening
Irregular antibody
Antigen spectrum
Electronic matching
Blood TransfusionSafety
