摘要
以新疆野生黑脉羊肚菌菌株为材料,在实验室中进行液体培养,对其在培养时的培养方式、最适pH值、光照条件、培养基及其营养物质进行了初步研究。试验结果表明,在棕色瓶内进行旋转式摇瓶培养是最佳培养方式,最适pH值为7.5,最适培养基为4,即黄豆芽200 g(煮汁)、蔗糖20 g、玉米粉5 g、水1 000 mL。
Taking the strain of Xinjiang Morchella angusticeps Peck as material, we primary studied its optimum liquid culture conditions, including suitable culture way, pH value, light condition, medium component and so on. The results showed that, the biological yield of mycelium was highest while the strain was cultured in rotary brown shake flask, and the most suitable pH value was 7.5. Medium 4, with 200 g soybean sprouts (boiled to juice), 20 g sucrose, 5 g corn flour and 1 000 mL sterilized water, was optimum for the mycelium growth of Xinjiang M. angusticeps Peck.
出处
《长江蔬菜》
2013年第16期71-74,共4页
Journal of Changjiang Vegetables
关键词
黑脉羊肚菌
液体培养
菌丝
Morchella angusticeps Peck
Liquid culture
Mycelium