摘要
以仙客来(Cyclamen persicum Mill.)开花植株的新生叶片为外植体,诱导筛选胚性愈伤组织,并使其进一步发育成体细胞胚。组织切片观察结果表明:胚性愈伤组织由胚性和非胚性细胞组成,胚性细胞多以胚性细胞团的形式存在,胚性细胞团起源于诱导的胚性决定细胞。体细胞胚起源于单个胚性细胞,经多细胞原胚、球形胚、心形胚和鱼雷胚等时期发育成完整植株。在体细胞胚的发生发育过程中,淀粉粒出现4次积累高峰,分别为胚性细胞、球形胚、早期鱼雷胚和成熟胚发育成完整的小植株时期,淀粉代谢与体细胞胚发生、发育及小植株的形态建成密切相关。
Embryogenic calluses were induced and filtrated from young leaf explants in flowering Cyclamen persicum plant,and somatic embryos were formed. Histological observation revealed that embryogenic calluses were comprised of embryogenic and non-embryogenic cells,and that embryogenic cells mostly existed in embryogenic aggregates. Embryogenic aggregates derived from induced embryogenic determined cells. Somatic embryogenesis originated from a single embryogenic cell. Somatic embryo grew into a complete plantlet after passing through proembryos,globular,heart- and torpedo- shaped stages. During somatic embryogenesis and development,four peaks of starch granules appeared, respectively at the stage of embryogenic cells,globular embryos,early torpedo-shaped embryos and complete plantlets from mature embryos. Starch metabolism was closely related to somatic embryogenesis,development and plantlet regeneration.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第8期1527-1534,共8页
Acta Horticulturae Sinica
基金
烟台市科技发展计划项目(2011045)
山东省科技发展计划项目(2007GG20002019)
关键词
仙客来
体细胞胚
诱导的胚性决定细胞
淀粉粒
Cyclamen persicum
somatic embryo
induced embryogenic determined cell
starch granule
