摘要
转VHA-c2-GUS,VHA-c3-GUS和VHA-c5-GUS基因烟草在4℃和40℃温度胁迫以及25℃光/暗处理条件下叶片GUS表达水平表明,VHA-c具有光调控性,并可被逆境温度所调节.VHA-c2,VHA-c3和VHA-c5均可被4℃低温和光照促进表达.40℃高温可显著促进VHA-c3的表达,对VHA-c5有轻微的促进,而对VHA-c2则表现出轻微的抑制作用.
To analyze the promoter activity of VHA-c genes of Arabidopsis thaliana, The GUS repoter gene was fused with the promoter of VHA-c2, c3 and c5 respectively, and the VHA-c-GUS constructs were transformed into tobacco leaves. Expression analysis of GUS activity under 4 ℃, 40 ℃ or 25 ℃ showed that all the three promoters were induced by low tem- perature, while the VHA-c3 promoter was up-regulated under 40 ℃, but the VHA-c2 promoter was inhibited slightly under the same conditions. Light also up-regulated GUS expression driven by the three promoters respectively, indicating that all the three promoters were responsive to light.
出处
《河南师范大学学报（自然科学版）》
CAS
北大核心
2013年第4期120-123,共4页
Journal of Henan Normal University(Natural Science Edition)
基金
国家自然科学基金(30460018)
