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拟南芥VHA-c3基因的特异性表达和调节 被引量：5

Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana
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摘要 利用RT-PCR技术检测VHA-c基因在拟南芥中的表达，结果表明VHA-c3基因在拟南芥的果荚、花、叶、茎和根中都有表达，但是，在叶中的表达量远远高于其它的组织．以GUS基因作为报告基因构建了不同长度的VHA-c3基因启动子缺失突变体，利用农杆菌介导的瞬时表达系统检测GUS基因的表达，研究发现在VHA-c3基因起始密码子上游2812～2234bp之间的区域内存在着控制VHA-c3基因高表达的转录调控元件． Gene expression of VHA c was detected in Arabidopsis silique, flower, leaf, bolt, root. However, the expression level of VHA-c3 was much higher in leaves than in the other tissues. Analysis of the putative promoter of VHA-c3 was carried out in tobacoo leave system. A series of the promoter mutants were constructed in which various extents of VHA-c3 promoter were placed upstream of the GUS gene in vector pC1305.1. The expression of GUS was analyzed by Agrobacterium mediated transient expression system. It was found that a 2 812 bp promoter region has high gene expression capacity. And there is a high potential key cis element for leafspecific expression between 2 812-2 234 bp region.
作者 徐萍 李小方 曾卫军 王水平
机构地区 华东师范大学生命科学学院
出处 《华东师范大学学报（自然科学版）》 CAS CSCD 北大核心 2006年第2期98-104,111,共8页 Journal of East China Normal University(Natural Science)
基金 国家自然科学基金资助项目(30460018)
关键词 V-ATPASE C亚基 VHA-c3基因 启动子 V-ATPase c subunit VHA c3 promoter
分类号 Q945 [生物学—植物学]
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参考文献11

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同被引文献41

  • 1徐萍,刘瑞香,曾卫军,李国婧,王瑞刚,王水平.拟南芥VHA-c基因在非生物胁迫响应中的作用[J].华北农学报,2006,21(1):19-22. 被引量：4
  • 2Sze H, Schumacher K, Mullier M L, et al. A simple nomenclature a complex proton pump-VHA genes encode the vacuolar H + -ATPase. Trends Plant Sci, 2002,7 (4) : 157 - 161.
  • 3Luttge U , Rataiczak R. The physiology, biochemistry and molecular biology of the plant vacuolar ATPase. Adv Bot Res , 1997 ,25:253 -296.
  • 4Rtaiczak R. Structure, function and regulation of the plant vacuolar H ^+ -translocating ATPase . Biochimica et Biophysica Acta, 2000 ,1465:17 -36.
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  • 7PereraI Y, Li X , Sze H. Several distinct genes encode nearly identical 16 kDa proteolipids of the vacuolar H ^+ -ATPase from Arabidopisis thaliana. Plant Mol Bio, 1995, 29 (2) : 227- 244.
  • 8Padmanaban S, Lin X, Perera I, et al . Differential expression of vacuolar H ^+ -ATPase subunit c genes in tissues active in membrane trafficking and their roles in plant growth as revealed by RNAi. Plant Physiol, 2004 , 134:1 - 13.
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引证文献5

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华东师范大学学报（自然科学版）
2006年 第2期

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