摘要
目的检测肿瘤干细胞标记物CD133和CD90在人原发性肝细胞癌组织中的表达情况,分析其与肝细胞癌的生物学行为及预后之间的关系。方法运用组织芯片和免疫组织化学(PV6000)方法检测245例肝细胞癌组织、82例癌旁组织和82例肝硬化组织中CD133、CD90的表达,分析其与临床病理特征和生存期的关系,并分析CD133与CD90表达的相关性。结果肝细胞癌组织中CD133的阳性表达率明显低于癌旁组织和肝硬化组织(χ2=71.736、21.497,P<0.05),且CD133表达与肿瘤分化程度有关,高分化肝细胞癌的阳性表达率明显高于低分化肝细胞癌(χ2=6.634,P<0.05)。肝细胞癌组织中CD90的阳性表达率明显高于癌旁和肝硬化组织,差异有显著性(χ2=42.201、42.201,P<0.05),且CD90表达与肿瘤大小相关(χ2=4.572,P<0.05)。CD133与CD90在肝细胞癌组织中的表达均与生存期无关。CD133与CD90在肝细胞癌组织中的表达无相关性。结论 CD133作为肝癌干细胞的标记物有待进一步探讨,CD90可能在肝细胞癌的发生、发展过程中发挥重要作用。
Objective To detect the expressions of tumor stem cell markers CD133 and CD90 in human primary hepato- cellular carcinoma （HCC）, and analyze the relationship between CD133 and CDg0 expression and biological behavior as well as prognosis of HCC. Methods BY using tissue microarray and immunohistochemical method （PV 6000）, the expressions of CD133 and CD90 were determined in HCC tissue of 245 cases, para-carcinoma tissue of 82 cases, and cirrhotic tissue of 82 cases. The relationship between the expressions and clinicopathological characteristics as well as survival was analyzed, the correlation be- tween CD133 and CD90 was judged as well. Results The expression of CD133 in the cancer tissue was much lower than that in para-carcinoma and cirrhotic tissue （X^2=71.736,21.497 ;P〈0.05）, and the expression was related to tumor differentiation, that in well differentiated HCC was higher than that in poorly differentiated HCC （X^2 = 6.634,P〈0.05）. CD90 expression was obviously hig-her in HCC tissue than in para-carcinoma and cirrhotic tissue （X^2 = 42. 201,42. 201;P〈0.05）. CD90 was correlated with the tumor size （X^2 =4. 572,P〈0.05）. The expressions of CD133 and CD90 in HCC were not associated with life span. There was no correlation between CD133 and CD90 in HCC tissue. Conclusion CD133 as a marker of liver cancer stem cells remains to be fur- ther discussed and CD90 is likely to play an important role in the development and progression of HCC.
出处
《青岛大学医学院学报》
CAS
2013年第5期393-396,共4页
Acta Academiae Medicinae Qingdao Universitatis
