摘要
目的 探讨转化生长因子 - β(TGF- β)在慢性阻塞性肺疾病 (COPD)支气管重构中的作用。方法 采用 SP免疫组织化学方法观察 TGP- β在 1 1例 COPD组纤维支气管镜标本组织的表达 ,并与 1 0例对照组比较。结果 (1 ) TGF-β在对照组支气管上皮细胞无表达 ,在 COPD组支气管粘膜上皮细胞有明显的表达并与对照组相比有显著的差异 ,支气管粘膜上皮细胞基底膜是主要阳性表达区 ;化生粘膜上皮有较多阳性细胞数 ,表达增强。 (2 ) TGF-β在对照组粘膜下层有较少阳性细胞表达 ,而在 COPD组表达增强 ,主要位于粘膜下层浸润的细胞、平滑肌细胞、粘膜腺体和内皮细胞。此外 ,TGF- β在细胞外基质和上皮下基底膜的胶原带有强表达。 (3) TGF-β表达强度与基底膜厚度和纤维细胞数呈正相关。结论 TGF-β可能参与 COPD气道重建时纤维化的形成过程 ,并在此过程中起重要作用。
Objective To explore the roles of transforming growth factor-β(TGF-β) in remodling of chronic obstructive pulmonary disease(COPD). Methods To investigate TGF-β expression in bronchial mucosal biopsies from 10 normal subjects and 11 patients with COPD by immunohistochemical staining.Results 1) TGF-β was rarely expressed in epithelial cells from controls, in comparison with the controls,there was a great and highly significant increase (P <0.001)in COPD.Positive staining was mainly observed on basal bronchial epithelial cells,and the number of poistive cells and the intensity of the staining was greater in metaplastic epithelium. 2) The number of cells expressing TGF-β was low in controsl and significantly greater in COPD(<0.001).The TGF-β immunostaining was localized on cells infiltrating the submucosa, on smoothmuscle cells, and on mucous glands and endothelial cells. Moreover, positive immunostaining for TGF-β was localized in subepithelial collagen zone of the basement membrane and in the extracellular matrix. 3) The increased expression of TGF-β is significantly correlated with the thickness of the basal membrane and the number of fibroblasts. Conclusion TGF-β may be involved in airways remodeling in COPD and play an important role in the pathogenesis of fibrotic alterations.
出处
《沈阳医学院学报》
2000年第3期141-143,共3页
Journal of Shenyang Medical College
关键词
生长因子
上皮细胞
慢性阻塞性肺疾病
growth factor
epithelial cell
chronic obstructive pulmonary disease(COPD)
