摘要
目的探讨硒和锌对小鼠成釉细胞DNA损伤的保护作用。方法以2．50、5．00、10．00μmol／L亚硒酸钠用于小鼠成釉细胞为硒作用组，以5．00、10．00、20．00μmol／L硫酸锌作用于小鼠成釉细胞为锌作用组。细胞作用24h后，采用单细胞凝胶电泳技术（SCGE）检测DNA单链断裂情况。结果与对照组比较，2．50和5．00μmol／L亚硒酸钠作用组小鼠成釉细胞DNA损伤的指标尾长、Olive尾矩、尾DNA％和尾长／头长值均明显下降（P〈0．05）；10．00μmol／L亚硒酸钠作用组小鼠成釉细胞的尾长、尾／头长比值较对照组均显著性降低（P〈0．05）。与对照组比较，5．00、10．00和20．00μmol／L硫酸锌作用组可使小鼠成釉细胞尾长和Olive尾距明显下降，差异均有统计学意义（P〈0．05）；5．00和10．00μmol／L硫酸锌作用组尾长／头长值明显下降（P〈0．05）。以Olive尾矩为观察指标，2．50和5．00μmol／L亚硒酸钠的保护作用要优于10．ooμmol／L亚硒酸钠，以2．50μmol／L亚硒酸钠保护作用相对较好；10．00和20.00μmol／L硫酸锌的保护作用要优于5．00μmol／L硫酸锌，以10．00μmol／L硫酸锌保护作用相对较好。结论2．50～10．0μmol／L的亚硒酸钠和5．00～20．00μmol／L硫酸锌均对成釉细胞DNA损伤具有一定的保护作用，其中2．50μmol／L亚硒酸钠和10．00μmol／L硫酸锌的相对保护作用较好。
[ Objective ] To investigate the protective effects of selenium and zinc against DNA damage in mouse ameloblasts. [ Methods] The mouse ameloblasts were treated with sodium selenite at the doses of 2.50, 5.00, 10.00 μmol/L, and zinc sulfate at the doses of 5.00, 10.00 and 20.00 μmol/L respectively. After exposed 24 h, the single cell gel electrophoresis assay （ SCGE ） was used to measure the DNA damage in mouse ameloblasts. [ Results ] The tail length, olive tail moment, DNA% and tail/head length ratio of sodium selenite group at the doses of 2.50, 5. 00 μmol/L were lower than those of the control group in mouse amelo- blasts （ P 〈 0. 05 ）. Selenium at the dose of 10. 00μmol/L sodium selenite could significantly decrease the value of the tail length and tail/head length ratio compared with the control （ P 〈 0. 05 ）. Compared with the control, zinc at the doses of 5.00, 10. 00 and 20. 00μmol/L zinc sulfate could significantly decreased the tail length and olive tail moment in mouse ameloblasts （ P 〈 0. 05 ） , the tail/head length ratio was significantly decreased in 5. 00 and 10. 00μmol/L zinc sulfate group （ P 〈0.05 ）. Selected olive tail moment as observation indicator, the protective effects of selenium at the doses of 2.50 and 5. 00μmol/L sodium selenite were better than that of 10. 00μmol/L sodium selenite, and also 10. 00and 20. 00μmol/L zinc sulfate better than that of 5. 00μmol/L zinc sulfate, especially for 10.00μmol/L sodium selenite. [ Conclusion] It is suggested that selenium at the dose of 2. 50-10. 0 μ,nol/L sodium selenite and zinc at the dose of 5.00-20.00μmol/L zinc sulfate can inhibit DNA damage in mouse ameloblasts respectively,especially for 2.5 μmol/L sodium selenite and 10. 00μmol/L zinc sulfate.
出处
《职业与健康》
CAS
2013年第17期2171-2174,共4页
Occupation and Health
基金
2010年广东药学院大学生创新实验项目
广东省自然科学基金(项目编号:9151022401000003)
