摘要
目的 研究氟对人胎肝细胞 (L 0 2细胞 )细胞周期、凋亡及p5 3表达的影响。方法 体外培养的人胎肝细胞接触 4 0、 80、 16 0 μg/ml氟化钠 2 4h后 ,MTT法检测L 0 2细胞存活率 ,流式细胞术检测细胞凋亡率和周期构成比 ,Westernblot检测P5 3蛋白的表达水平。结果 高剂量氟染毒组细胞存活率显著低于对照组 (P <0 0 1) ;各染氟组S期细胞数与对照组相比均明显增多 (P <0 0 1) ,中、高剂量氟染毒组细胞凋亡率和p5 3蛋白表达量均显著高于对照组 (P <0 0 1)。结论 氟可使L 0 2细胞S期细胞数增多 ,诱导人胎肝细胞凋亡和 p5 3表达 ,并呈剂量 效应关系。
Objective To investigate the effects of fluoride on cell cycle, apoptosis, and the level of p53 expression in human embryo hepatocytes (L-02 cells). Methods After the cultured human embryo hepatocytes (L-02 cells) were incubated with 40, 80, and 160 _g/ml sodium fluoride for 24 h in vitro, the survival rate of L-02 cells was measured by MTT method, apoptotic rate and cell cycle by flow cytometry, and the level of p53 expression detected by Western blot, respectively. Results The survival rate of L-02 cells treated by high dose of fluoride was significantly lower than that in the control group (P<0.01). Compared with the control group, the cell number in S phase was significantly increased in cells exposed to different doses of fluoride (P<0.01). The percentage of apoptotic rate and the level of p53 protein expression in middle and high doses of fluoride group were markedly higher than in control group (P<0.01). Conclusion Fluoride can increase the cell number in S phase, and induce apoptosis and p53 expression in L-02 cells in a dose-effect manner.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第5期550-552,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目 (No .30 2 7115 5 )
