摘要
提取中国甜杏仁过敏原蛋白苦杏仁球蛋白,分别制备兔和鼠多克隆抗体,建立双抗体夹心酶联免疫吸附(ELISA)法。结果表明:对甜杏仁中苦杏仁球蛋白的检测限为(6.36±1.02)μg/L;对中国苦杏仁及美国大杏仁中苦杏仁球蛋白的检测限分别为(12.11±1.70)μg/L和(18.95±1.52)μg/L,且与常见的14种植物性蛋白没有交叉反应,表明该方法具有良好的特异性。将该方法用于法式小面包、饼干和脱脂牛乳中杏仁过敏原的检测,苦杏仁球蛋白的添加回收率为78.94%～125.15%,且相对标准偏差均低于5.81%。
In the present study,allergen amandin from Chinese sweet almond was prepared,and specific antibodies against the amandin were produced.A double-antibody sandwich enzyme linked immunosorbent assay（ELISA） based on rabbit polyclonal antibody and mouse polyclonal antibody was then established.The newly established ELISA could detect amandin at levels as low as（6.36 ± 1.02） μg/L,showing no cross-reactivity with 14 common plant proteins.The limit of detection for amandin was（12.11 ± 1.70） μg/L in Chinese bitter apricot kernel and（18.95 ± 1.52） μg/L in almond（AmygdaluscommunisL.）.The sandwich ELISA were used to detect the amandin in spiked samples of Frenchstyle bread,biscuit and skim milk with recoveries ranging from 78.94% to 125.15%,and relative standard deviation（n = 3） below 5.81%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第16期173-177,共5页
Food Science
基金
"十二五"国家科技支撑计划项目(2011BAK10B03)
天津市科技计划项目(10SYSYJC28300)
关键词
杏仁
苦杏仁球蛋白
双抗体夹心酶联免疫吸附分析法
apricot kernel
amandin
double-antibody sandwich enzyme linked immunosorbent assay（sandwich ELISA）
