摘要
为了探索快速有效的苦瓜皂苷提取方法,该文利用响应面法研究了超声功率(133～167W/g)、乙醇体积分数(60%～80%)、十二烷基硫酸钠(SDS)质量浓度(15～25mg/mL)对苦瓜皂苷萃取率的影响,并对所得苦瓜皂苷的结构进行初步鉴定。结果表明响应面所建立的苦瓜皂苷提取模型能很好地预测不同提取条件下的苦瓜皂苷提取率。超声功率、乙醇体积分数、SDS质量浓度均对苦瓜皂苷提取率存在显著影响(p<0.05),其中超声波功率与SDS质量浓度间存在协同作用。验证试验表明超声功率为153W/g,乙醇体积分数为74%,SDS质量浓度为16mg/mL,苦瓜皂苷提取率达到3.22%,显著高于(p<0.05)乙醇及超声萃取。液相色谱-多级质谱表明所萃取的皂苷由苦瓜皂苷L和苦瓜皂苷F2组成。超声波-表面活性协同萃取是一种有效的提取皂苷方法,值得进一步的开发应用。
Balsam pear （Momordica charantia L.）, a climber belonging to family Cucurbitaceae, is commonly known as bitter gourd or bitter melon in English. It is cultivated throughout the world for use as vegetable as well as medicine. Balsam pear has been used traditionally as medicine in developing countries. Some of its common uses in most countries are for diabetes, a carminative and in treatment of colics. Topically it is used for treatment of wounds, internally as well as externally for management of worms and parasites. It is also used as emmenagogue, antiviral for measles and hepatitis. Balsam pear contains biologically active chemicals that include glycosides, saponins, alkaloids, fixedoils, triterpenes. Saponins from all parts of the plant （fruit pulp, seed, leaves and whole plant） are most widely studied with regard to its antidiabetic effect. Ultrasound and surfactants have been individually shown to enhance saponins extraction. In this study, we investigated the synergistic effect of ultrasound and surfactants on saponins extraction. In order to explore effective extracting method of saponins from balsam pear, response surface was used to study the effect of ultrasonic power （133-167 W/g）, ethanol volume fraction （60%-80%） and mass concentrations of SDS （15-25 mg/mL） on extraction rate of saponins from balsam pear. Ultrasound-surfactant synergistic extraction was compared with ethanol and ultrasound, respectively. The structures of the prepared saponins from balsam pear were identified. Results showed that the established model of extraction could well predict extraction rate of saponins from balsam pear under different conditions. Extraction rate of saponins from balsam pear was significantly （p0.05） influenced by ultrasonic power, ethanol volume fraction and mass concentrations of SDS, and there was synergistic effect between ultrasonic power and the mass concentration of SDS. Various factors affecting extraction rate of saponins from balsam pear in a descending order was as follows： mass concentrations of SDS, ultrasonic power, ethanol volume fraction. Extraction rate of saponins from balsam pear was 3.22% at ultrasonic power of 153 W/g, ethanol volume fraction of 74%, mass concentrations of SDS of 16 mg/mL, significantly higher （p0.05） than that of ethanol and ultrasound extraction. Liquid chromatography-mass spectrometry revealed that extracted saponins included momordicoside L and F2 . Ultrasound-surfactant synergistic extraction is an effective method for extraction of saponins, which is worth further development and application.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2013年第15期294-300,共7页
Transactions of the Chinese Society of Agricultural Engineering
基金
国家农业科技成果转化资金项目(2008GB24420474)
关键词
超声波
优化
模型
苦瓜皂苷
表面活性剂
协同萃取
结构鉴定
ultrasonic waves
optimization
models
saponins from balsam pear
surfactant
synergic extraction
structure identification
