摘要
目的 从细胞及组织水平检测血小板源性生长因子受体 - β(PDGFR- β)在正常皮肤及瘢痕疙瘩中的表达及分布 ,探讨 PDGFR- β在瘢痕疙瘩形成中的生物学作用。方法 1应用免疫组织化学技术检测 PDGFR-β在正常皮肤和瘢痕疙瘩组织中的分布 ;2应用免疫荧光、流式细胞仪检测正常皮肤和瘢痕疙瘩成纤维细胞群体的PDGFR- β的表达 ;3应用免疫荧光、激光共聚焦显微镜检测 PDGFR- β的亚细胞分布。结果 1免疫组织化学 正常皮肤与瘢痕疙瘩组织中 PDGFR- β表达基本相同 ;2流式细胞仪检测 正常皮肤成纤维细胞 PDGFR- β的表达明显高于瘢痕疙瘩成纤维细胞 ,有统计学意义 (P<0 .0 1) ;3激光共聚焦显微镜检测 在正常皮肤成纤维细胞中PDGFR- β主要集中于细胞核膜及核内 ,瘢痕疙瘩成纤维细胞中 PDGFR- β主要集中于细胞膜。结论 1体外培养的成纤维细胞和组织中成纤维细胞 PDGFR- β有差异 ;2正常皮肤成纤维细胞 PDGFR- β表达高于瘢痕疙瘩成纤维细胞 ;3正常皮肤成纤维细胞与瘢痕疙瘩成纤维细胞中 PDGFR- β亚细胞分布不同 ,这可能是瘢痕疙瘩形成的重要因素。
Objective To investigate the expression and distribution of platelet derived growth factor receptor-β(PDGFR-β) in normal skin and keloid and to discuss its biological function in keloid formation. Methods ① To detect the expression and distribution of PDGFR-β in normal skin and keloid tissue by immunohistochemistry; ② To detect the receptor expression in vitro by Flow cytometry (FCM); ③ To detect the subcellular distribution of receptor by Laser confocal microscope. Results ① Immunohistochemistry showed that normal skin and keloid tissue were almost the same in expression but different in distribution of PDGFR-β; ②There was more expression of PDGFR-β in normal fibroblasts than that in keloid fibroblasts in vitro by FCM; ③ Laser confocal microscope revealed that the PDGFR-β concentrated on the surface of cell membrane in keloid fibroblasts, but in normal skin fibroblasts, the receptors were coaggulated on the nuclear membrane and intranucleus. Conclusion Compared with the fibroblasts in vivo , there was a difference of the PDGFR-β expression in fibroblasts in vitro , more expression of PDGFR-β in normal fibroblast than that in keloid fibroblast in vitro ; and the subcellular distribution of PDGFR-β was different in normal skin and keloid fibroblasts. The characteristics of the expression and distribution of PDGFR-β in keloid may contribute to the formation of keloid.
出处
《中国修复重建外科杂志》
CAS
CSCD
2000年第5期278-282,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金!资助项目 (39970 75 9)
关键词
瘢痕疙瘩
PDGFR-Β
成纤维细胞
表达
Keloid
Platelet derived growth factor receptor
Laser confocal microscope
