摘要
目的探讨胰岛素样生长因子-1(IGF-1)对缺氧缺血性脑损伤(HIBD)急性期新生小鼠脑细胞线粒体膜电位(ΔΨm)的影响。方法采用改良Rice方法制备新生小鼠HIBD动物模型。7日龄C57/BL新生小鼠91只随机分为正常对照组(n=7)、假手术组(n=22)、缺氧缺血组(HI组,n=22)、假手术+IGF-1组(n=18)、HI+IGF-1组(n=22)。假手术+IGF-1组、HI+IGF-1组在术后即刻予腹腔注射IGF-1(50μg/kg),假手术组、HI组注射等体积生理盐水,各组于术后0、3、6、12、24、48 h取大脑皮质、海马、丘脑,制成单细胞悬液以罗丹明123(Rho123)标记后使用流式细胞仪测定脑细胞线粒体膜电位。结果①皮质、海马、丘脑分别在术后3、6、12 h出现肿胀、凋亡,HI后48 h神经元肿胀、变性最明显,具有凋亡特征神经细胞达高峰;②正常对照组、假手术组、假手术+IGF-1组皮质、海马、丘脑各部位在不同时点平均荧光强度差异无统计学意义;③IGF-1对大脑皮质损伤修复有两个间期,对丘脑修复延迟且作用时间短;④HI组脑细胞平均荧光强度的升高最先出现在皮质(术后3 h),其次为海马(术后6 h),最后在丘脑(术后12 h),HI+IGF-1组各部位脑细胞平均荧光强度低于HI组(P<0.01)。结论IGF-1可以减轻HIBD后脑水肿,减少神经细胞凋亡;推测IGF-1的神经保护作用可能与减轻HIBD急性期新生小鼠各部位脑细胞线粒体膜电位的降低、改善线粒体功能有关。
Objective To investigate the effect of insulin-like growth factor-1（IGF-1） on hypoxic ischemic brain damage（HIBD） in acute phase of newborn mice brain mitochondria membrane potential.Methods Using modified Rice method to establish newborn HIBD animal model,91 7-day-old C57/BL newborn mice were randomly divided into normal control group（n=7）,sham group（n=22）,sham＋IGF-1 group（n=18）,HI group（n=22）,HI＋IGF-1 group（n=22）.The normal control group did not deal with it,and the sham group just separated the bilateral common carotid artery.After the operation,the two groups（sham＋IGF-1 group,HI＋IGF-1 group） immediately were injected IGF-1 50 μg/kg,and sham group and HI group were injected the same amount of saline solution.Respectively on postoperative 0,3,6,12,24,48 h the mice were execated and cerebral cortex,hippocampus,thalamencephalon were taken out and digested into single cell suspension,Rhodamine123 dyed and used flow cytometry to detect the mitochondrial membrane potentia.Results ① The cortex,hippocampus,thalamus respectively on postoperative 3,6,12 h began to appear edema,apoptosis,48 h after HI the neurons were swelling,degenerated significantly and the apoptosis ones were peaked.② The normal control group,sham group,sham＋IGF-1 group of newborn mice cerebral cortex,hippocampus,thalamus at different time points on mean fluorescence intensity had no obvious differences.③ That IGF-1 repaired the brain cortex injury had two interphase,thalamus repaired delayed and acted within a short time.④ Mean fluorescence intensity went up in order of cortex,hippocampus,thalamus respectively on postoperative 3,6,12 h,but HI＋IGF-1 group were lower than HI group.Conclusion IGF-1 can relieve HIBD caused cerebral edema,reduce the nerve cell apoptosis.Nerve protective effect of IGF-1 may be associated with the fact that IGF-1 can reduce newborn mice brain mitochondrial membrane potential in the acute phase of HIBD and improve mitochondrial function.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第9期1001-1005,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金面上项目(编号:1308085MH150)
安徽省高等学校省级自然科学研究项目(编号:KJ2013A167)
关键词
缺氧缺血
脑
线粒体膜电位
胰岛素样生长因子-1
小鼠
hypoxia-ischemia
cerebral
mitochondrial membrane potential
insulin-like growth factor-1
mice
