摘要
以乙醇和水作为提取溶剂,用微波炉提取金银花中的绿原酸,用紫外可见分光光度计对金银花中绿原酸的浓度进行测定,采用单因数测定和正交实验得出金银花中绿原酸最佳提取工艺。测定波长选择324 nm,实验表明,金银花中绿原酸的最佳微波提取条件为:以浓度为80%的乙醇做为提取溶剂,加醇量为金银花4倍量,微波处理时间为60 s,水浴提取温度为70℃,提取水的用量为金银花15倍量,提取时间为50 min。得出金银花中绿原酸的含量为8.3379 mg.g-1。
Abstract： Chlorogenic acids were extracted from Lonicera japonica by microwave oven with ethanol and water as the extrac- tion solvents. The concentration of the chlorogenic acids was detected by UV - vis spectrophotometer with the set wavelength at 324nm. The experiment shows that the optimal extraction conditions of microwave extraction of chlorogenic acid from Flos Lonicerae is ： with the concentration of 80% ethanol as extraction solvent, alcohol amount for honeysuckle 4 times amount, micro- wave treatment time is 60 s, water bath extraction temperature is 70 ℃, the amount of water as extracting honeysuckle 15 times, extraction time 50 min. The results show that the content of chlorogenic acid in Flos Lonicerae is 8. 3379 mg · g^-1.
出处
《湖南人文科技学院学报》
2013年第4期97-101,共5页
Journal of Hunan University of Humanities,Science and Technology
基金
湖南省大学生研究性学习和创新性实验计划项目"湘中地区金银花中绿原酸的测定及提取与纯化方法优化研究"(5411210)
关键词
金银花
绿原酸
微波
提取
honeysuckle
chlorogenic acids
microwave
extract
