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新型低氧还原剂Q39通过阻断HIF-1转运引起肝癌Bel-7402细胞凋亡 被引量：1

Novel Hypoxia-slective Compound Q39 Induced Bel-7402 Cell Apoptosis via Blocking HIF-1 Translocation
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摘要 目的评价Q39低氧条件下诱导肝癌细胞Bel-7402细胞凋亡的抗肿瘤活性及其机制。方法 MTT法测定Q39对人肝癌细胞Bel-7402增殖抑制作用。PI染色法检测Q39诱导人肝癌细胞凋亡作用。免疫荧光法检测HIF-1蛋白的转运和表达。结果 Q39在常氧和低氧下均抑制肝癌Bel-7402细胞的生长。Q39在低氧条件下促进Bel-7402肿瘤细胞凋亡。Q39通过抑制ERK1/2的磷酸化,明显抑制HIF-1的转运。结论 Q39在低氧条件下通过抑制ERK1/2信号通路引起Bel-7402肿瘤细胞凋亡。 OBJECTIVE To study the anti-cancer activity and mechanism of Q39 in hypoxia.METHODS The anti-proliferation activity of Q39 were analyzed by MTT,apoptosis was detected by PI and flowcytometry analysis.HIF-1 protein expression level and translocation was analyzed by immunostaining and WB.RESULTS The present study indicated that Q39 exerted anti-proliferative effects against human cancer cells Bel-7402 in hypoxia.Downregulation of ERK1/2 inhibited by Q39 resulted in blocking HIF-1 translocation,which further induced the apoptosis of Bel-7402.CONCLUSION These findings build the rationale for further development of candidate compound Q39 against hepatoma（Bel-7402）.
作者 邵燕飞 俞佳 朱虹
机构地区 浙江大学药学院药理毒理与生化药学研究所 浙江省人民医院药剂科临床药学室
出处 《中国现代应用药学》 CAS CSCD 2013年第7期718-722,共5页 Chinese Journal of Modern Applied Pharmacy
关键词 Q39 低氧诱导因子HIF-1 ERK1 2 细胞凋亡 抗肿瘤活性 Q39 HIF-1 ERK1/2 apoptosis anti-tumor activity
分类号 R965.1 [医药卫生—药理学]
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参考文献11

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同被引文献64

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中国现代应用药学
2013年 第7期

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