摘要
检索橡胶树EST和基因组数据库,发现了1个在叶片中高丰度表达的中碱性转化酶基因。采用RT-PCR和RACE技术获得了该基因的cDNA和基因组序列,命名为HbNINa。序列分析结果显示,HbNINa基因cDNA序列的编码区(CDS)序列长度为1 719 bp,对应的基因组序列为3 696 bp。序列分析表明,该基因编码571个氨基酸多肽,推测其分子量大小为65.7 ku,等电点为6.36。HbNINa蛋白包含植物中碱性转化酶的全部保守结构域。基因组结构分析显示HbNINa基因包含4个外显子和3个内含子。QPCR分析结果表明,HbNINa基因在在胶乳中表达水平极低,而在其他组织如树皮、树叶和雌花中的表达丰度相对较高。在叶片发育过程中,HbNINa在叶片发育的初期高丰度表达,推测HbNINa可能参与叶片蔗糖利用,进而在橡胶树叶片发育过程中起重要作用。
Based on the Hevea EST and genome sequence database,we cloned one alkaline/neutral invertase gene,HbNINa,which was highly expressed in the leaf of Hevea brasiliensis.Sequence analysis revealed that HbNINa coding sequence was 1 719 bp,and the deduced peptide was 571 amino acids in length,with an average molecular weight of 65.7 ku and a pIvalue of 6.36.The HbNINa protein contained all the conserved domains of typical plant alkaline/neutral invertases.Exon/intron structure analysis showed that the HbNINa gene contained four exons and three introns.The real-time RT-PCR results indicated that the HbNINa expression was mainly found in the bark,leaf and female flower,whereas barely detectable in the latex.HbNINa was also found highly expressed in bronze-stage leaf and down-regulated with leaf maturation,suggesting the important roles of this gene in the process of leaf maturation in rubber tree.
出处
《热带作物学报》
CSCD
北大核心
2013年第7期1264-1269,共6页
Chinese Journal of Tropical Crops
基金
国家自然科学基金计划项目(No.31170630)
海南省自然科学基金项目(No.310094)
国家863课题(No.2013AA102605)
关键词
巴西橡胶树
中碱性转化酶
基因结构
表达分析
叶片发育
Hevea brasiliensis
Alkaline/neutral invertase
Gene structure
Expression analysis
Leaf development
