摘要
采用四甲基偶氮唑盐(MTT)比色法检测超声辅助提取后经季铵盐沉淀分级得到的鸡腿菇多糖(UCP-3)、硫酸酯化鸡腿菇多糖(SWCP)和羧甲基化鸡腿菇多糖(CWCP)对人白血病K562细胞体外生长的抑制活性,研究不同的处理工艺对鸡腿菇多糖体外抑制K562细胞增殖活性的影响.结果表明:3种鸡腿菇多糖均可抑制K562细胞的体外生长,抑制作用随着多糖浓度和作用时间的变化而变化;超声处理多糖可能引起单糖之间连接方式变化进而使得其生物活性发生改变;SWCP与CWCP对K562细胞的体外生长抑制作用明显,所以对鸡腿菇多糖进行化学修饰有助于提高其生物活性.
The in vitro anti-tumor activity of three kinds of Coprinus comatus polysaccharides were tested by MTT assay. The Coprinus comatus polysaccharides were isolated by ultrasonic （UCP-3）, then carboxymethylated Coprinus comatus polysaccharide （CWCP） and sulfated Coprinus comatus polysaccharide（SWCP） were obtained respectively. The analyses of anti-tumor activity of polysaccharides UCP-3, SWCP and CWCP on human leukemia K562 cells in vitro were carried out. The effect of in vitro anti-tumor activity of Coprinus comatus polysaccharides extracted through different processes was investigated. The results show that three kinds of Coprinus comatus polysaccharides all have inhibitory action on K562 cells. The ultrasound can induce the connection pattern change of monosaccharides thus resulting the change of bioactivity. The inhibition effect of SWCP and CWCP are obvious enhanced, so sulfation and carboxymethylation modifications can improve the biological activity.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第4期73-78,共6页
Journal of Shaanxi Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(10874108)
陕西省自然科学基础研究计划项目(SJ08A16)
关键词
鸡腿菇多糖
超声提取
硫酸酯化
羧甲基化
抗肿瘤活性
Coprinus comatus polysaccharide
ultrasound extraction
sulfation
carboxymethylation
invitronetivitv
