摘要
目的：探讨激光对体外培养瘢痕成纤维细胞胶原合成的选择性抑制作用。方法：以不同能量密度（５００Ｊ／ｃｍ~２、１０００ Ｊ／ｃｍ~２、１５００ Ｊ／ｃｍ~２和２０００ Ｊ／ｃｍ~２）Ｎｄ：ＹＡＧ激光照射体外培养增生性瘢痕和正常成纤维细胞，照射后２４ｈ分别用~３Ｈ－脯氨酸掺入法和斑点杂交检测成纤维细胞胶原合成和Ⅰ型前胶原基因表达。结果：培养的增生性瘢痕和正常皮肤成纤维细胞的胶原合成和 Ⅰ型前胶原ｍＲＮＡ相对量在 ５００ Ｊ／ｃｍ２激光照射后无明显改变（Ｐ＞０．０５），１５００ Ｊ／ｃｍ~２和 ２０００ Ｊ／ｃｍ~２照射后显著降低（Ｐ＜０． ００１），１０００ Ｊ／ｃｍ~２能量密度激光照射后增生性瘢痕成纤维细胞的胶原合成与Ⅰ型前胶原基因表达显著下降（Ｐ＜０．００１），而正常皮肤成纤维细胞的胶原合成和Ⅰ型前胶原基因表达无变化。结论：以１０００Ｊ／ｃｍ~２脉冲Ｎｄ：ＹＡＧ激光照射能选择性抑制增生性瘢痕成纤维细胞的胶原合成和Ⅰ型前胶原基因表达。
To study the effects of pulsed Nd: YAG laser irradiation on collagen production of scar fibroblasts. Methods: Cultured fibroblasts derived from hypertrophic scars (HS) or normal human skin were treated with pulsed Nd: YAG laser at various energy density levels (500 J/cm2, 1000 J/cm^2, 1 500 J/cm^2 and 2000 J/cm^2). 24 hours after laser irradiation, collagen production of the fibroblasts was measured by [~3H] probine incorporation. The expression of proα1(Ⅰ) procollagen mRNA was investigated by blot hybridization. Results: Collagen synthesis and type Ⅰ procollagen mRNA level in HS and normal dermal fibroblasts were not significantly different at energy level of 500 J/cm^2 (P>0. 05),and were significantly decreased at energy densities of 1 500 J/cm^2 and 2 000 J/cm^2 (P<0. 001). Colloagen production and type Ⅰ procollagen level in HS fibroblasts were markedly reduced at energy level of 1 000 J/cm^2 (P<0. 001),while the doses did not affect collagen synthesis and type Ⅰ procollagen mRNA level in normal dermal fibroblasts. Conclusion: Pulsed ND: YAG laser at energy density of 1 000J/cm^2 can selectively suppress collagen synthesis and type Ⅰ procollagen mRNA level in HS fibroblasts.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2000年第4期257-260,共4页
Journal of Practical Stomatology
