摘要
目的：探讨增生性瘢痕（Ｈ）和瘢痕疙瘩（Ｋ）组织中Ⅰ、Ⅲ型前胶原及ＴＧＦ－β１基因表达改变的相互关系及临床意义。方法：总ＲＮＡ抽提试剂盒抽提总ＲＮＡ，斑点杂交检测Ⅰ、Ⅲ型前胶原及ＴＧＦ－β１ｍＲＮＡ稳态水平的改变。结果：Ｈ和Ｋ组织中ＴＧＦ－β１ｍＲＮＡ（１．１９７±０．２３７，１．２０４±０．２４３）表达均高于正常瘢痕和正常皮肤（０．３２７±０．０８１，０．３３１±０．０７８），Ｐ＜０．０１；Ｋ选择性地Ⅰ型前胶原ｍＲＮＡ表达增强，而Ｈ组织中Ⅰ、Ⅲ前胶原ｍＲＮＡ表达均增强，导致ＫⅠ、Ⅲ前胶原ｍＲＮＡ比值明显高于Ｈ（８．１６４±０．３００，１．６６６±０．２０１，Ｐ＜０．０１）。结论：Ｋ和Ｈ组织中胶原蛋白基因表达类型及强度不同，提示在Ｋ和Ｈ发生发展中具有不同的分子机理：ＴＧＦ－β１在增生性瘢痕疙瘩的发病机理中具有不同的分子机理；ＴＧＦ－β１在增生性瘢痕和瘢痕疙瘩的发病机理中具有一定作用。
Objective:To investigate the correlation between pathogenesis and expression of typeⅠ、Ⅲ Procollagen and TGF-β 1 mRNA in keloid and hypertrophic scar tissue. Methods: total RNA was extracted with TRIZOL TM Regent. Dot-blot hybridization was used to test the change of type Ⅰ、Ⅲ procollagen and TGF-β 1 mRNA in tissue samples. Results: the expression level of mRNA for TGF-β 1 increased significantly in keloid and hypertrophic scar compared with normal scar and normal skin.( P <0.001);In keloid tissue, type I procollagen mRNAs expression increased selectively. However, type I and Ⅲ collagen mRNAs expression increased in hypertrophic scar. There was a significantly positive correlation between pro α 1( Ⅰ) and TGF-β 1 mRNA expression(r=0.9113, P <0.001).Conclusion: Keloid and hypertrophic scar tissues had different type and intensity of collagen gene expression. It suggested that distinct molecular mechanisms were operative in the development of keloid and hypertrophic scar; TGF-β 1 played an important role in the pathogenesis of keloid and hypertrophic scar.
出处
《中国现代医学杂志》
CAS
CSCD
1999年第5期8-9,11,共3页
China Journal of Modern Medicine
