摘要
目的 :观察加味地黄汤对骨科创伤患者创伤早期免疫功能抑制的调理作用。方法 :用流式细胞检测术测定患者外周血 T细胞亚群 ,用单向免疫扩散法测定患者外周血补体 C3 、免疫球蛋白 Ig G、Ig A、Ig M和C反应蛋白 ( CRP)水平。结果 :创伤后 2 4小时内 ,患者外周血总 T细胞百分率 ( CD3 + % )、Th细胞百分率( CD4 + % )和 CD4 + / CD8+比值均降低 ,而 Ts细胞百分率 ( CD8+ % )则无明显异常改变 ,Ig G、Ig A、Ig M、C3 处于低水平 ,CRP水平却显著升高。治疗后第 6天 ,加中药治疗组患者外周血 CD3 + %、CD4 + %、CD4 + / CD8+ 比值、Ig G、Ig A、Ig M,C3 ,CRP与治疗前相比均有显著性差别 ( P<0 .0 1 ) ;基础治疗组患者与治疗前相比仅CD4 + %、CD4 + / CD8+比值、Ig A和 Ig M有显著性差别 ,CD3 + %、Ig G、C3 、CRP均无显著性差别 ;两组患者的CD8+ %与治疗前相比均无显著性差别。
Objective:To observe the regulating effect of Jiawei Dihuang (JWDH) decoction on the early immunosuppresive of injured patients.Methods: Twenty injured patients were divided at random into two groups. The peripheral blood T lymphocyte subsets of the patients were detected by flow cytometry, the complement C 3, immune globulin IgG, IgA, IgM and C reaction protein (CRP) were measured by single radical immunodiffusion test.Result:In the 24 hours post trauma, the percent of total T lymphocyte in the peripheral blood (CD3 +%), the percent of Th cell (CD4 +%) and the ratio of CD4 +/CD8 + declined respectively. However,the percent of Ts cell (CD8 +%) had no significantly change.The contain of C 3,IgG,IgA and IgM stood a lower level,on the contrary, the contain of CRP rose remarkably. In the sixth day after treatment, compared with those of six day before, the contain of CD3 +%, CD4 +%, CD4 +/CD8 +, IgG, IgA, IgM, C 3 and CRP in the peripheral blood of JWDH taking group had a significant rise (P<0.01); In basic treating group, only the contain of CD4 +%, CD4 +/CD8 +, IgA, IgM, C 3 had a significant rise (P<0.01); There is no significant change of the CD8 +% level in both groups.
出处
《中国中医骨伤科杂志》
CAS
2000年第4期21-24,共4页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
研究生课题
