摘要
目的 探讨创伤骨折及手术过程中细胞间粘附分子 1(ICAM 1)和血管细胞粘附分子 1(VCAM 1)的含量变化 ,了解创伤骨折手术后是否感染以及与创伤程度的相关性。方法 采用ELISA酶联免疫吸附法对 5 0例患者在创伤骨折及手术前后ICAM 1和VCAM 1的含量进行测定。以创伤时间进行分组 ,其中 ,创伤骨折后 90分钟内进行手术的患者 2 1例 ;骨折后 1～ 3天手术的患者 2 9例 ;正常对照组 30例。结果 创伤骨折后 90分钟进行手术的 2 1例患者血清ICAM 1和VCAM 1的含量与正常对照组的含量相比无显著性差异。而创伤骨折后 1～ 3天手术的患者 ,检测ICAM 1和VCAM 1的含量明显高于正常对照组和手术前的含量 ,有显著性差异。创伤骨折及手术后发生继发感染的 12例患者的ICAM 1和VCAM 1的含量明显高于手术后未发生感染组的含量 ,有显著性差异。结论 创伤骨折及手术能使机体的各种机能发生改变 ,淋巴细胞和巨噬细胞释放一些细胞因子 ,并可使内皮细胞和成纤维细胞表达ICAM 1和VCAM 1的功能增强。在创伤骨折及手术后发生继发感染后 ,体内白细胞游走更是依赖于I CAM 1和VCAM
Objective To determine the contents of ICAM 1 and VCAM 1 in patients with traumatic fracture and during the course of operation,in order to detect if infection occurred after operation and its relationship with the local conditions of the wound [HT5”H]Methods [HT5”SS]The contents of ICAM 1 and VCAM 1 in 50 patients suffered from fracture and underwent operation were detected by using Enzyme linked immunoadsordent assay(ELISA).The patients were divided according to the time of injury:group 1 of 21 cases had surgery within 90 minutes after fracture,and group 2 of 29 within 1～3 days.Group 3 of 30 normal persons served as control group.Results The contents of ICAM 1 and VCAM 1 in group 1 had no obvious difference from that of control group.While the contents of ICAM 1 and VCAM 1 in group 2 were higher than that of control group and before operation.The contents of ICAM 1 and VCAM 1 in 12 cases in which infection occurred after fracture and operation were much higher than that in patients with infection.Conclusion Trauma fracture and operation can cause release of cytokine by lymphocyte and macrophage,high expression of ICAM 1 and VCAM 1 by endodermis cells and fibroblast.After trauma and operation,when secondary infection occurred,the migration of white blood cell(WBC) is especially depended on participation of ICAM 1 and VCAM 1.
出处
《中国骨伤》
CAS
2001年第7期403-404,共2页
China Journal of Orthopaedics and Traumatology
关键词
细胞粘附分子
骨折
创伤
损伤
Cell adhesion molecules Fracture Trauma and injury
