摘要
目的观察胃癌患者癌组织及与其配对的正常胃黏膜组织中自细胞介素-8（IL-8）基因启动子甲基化及其mRNA表达，探讨IL-8基因甲基化与胃癌的关系。方法收集经病理确诊并行外科手术切除的胃癌组织及配对的正常胃黏膜组织各52例，用实时荧光定量聚合酶链反应（FQ—PCR）分别检测癌组织及正常组织中IL-8基因mRNA表达；用甲基化特异性PCR（MSP）检测上述两种组织中IL-8基因甲基化状态。结果胃癌组织中IL-8基因mRNA的表达量是正常组织中的1．94倍（P〈0．01）。胃癌组织中IL-8基因甲基化率为32．7％，而正常组织中的甲基化率为73．1％（P〈0．01）；胃癌组织中IL-8基因mRNA表达及启动子的去甲基化与性别、年龄、肿瘤大小无明显相关（P〉0．05），而与肿瘤的分化程度、Borrmann分型、浸润程度及有无淋巴结转移均具有明显相关（P〈0．05）；去甲基化的胃癌组织中IL-8mRNA表达是甲基化胃癌组织的2．13倍（P〈0．001）。结论IL-8基因mRNA在胃癌组织中过表达；胃癌组织中IL-8基因启动子区呈低甲基化状态；IL-8基因启动子区去甲基化促进了其在胃癌组织中高表达。
Objective To investigate the promoter methylation status and mRNA expression of in- terleukin-8 （IL-8） in gastric cancer tissue and paired normal tissue, and to explore the relationship be- tween IL-8 promoter methylation and gastric cancer. Methods The gastric cancer tissues （ n = 52） and paried normal tissues （n = 52） confirmed pathologically and resected surgically were collected, and the mRNA expression of IL-8 was detected by using real-time fluorescent quantitative polymerase chain reaction （FQ-PCR） and methylation status of the IL-8 gene by methylation-specific PCR （MSP）. Results There was significant difference in IL-8 gene mRNA expression and methylation status between normal gastric mu- cosa and gastric cancer （P 〈 0. 01 ）. The IL-8 gene mRNA expression and methylation status in gastric cancer were not significantly correlated with gender, age and tumor size （ P 〉 0. 05 ）, but significantly with degree of differentiation, Borrmann type, degree of infiltration and lymph node metastasis （ P 〈 0. 05）. There was also significant difference in the IL-8 mRNA expression between methylated gastric cancer tissue and unmethylated gastric cancer tissue （ P 〈 0. 01）. Conclusion The hypomethylation of IL-8 gene pro- moter promotes the over-expression of IL-8 mRNA in gastric cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第8期1730-1733,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（31270532、30870364）
兰州大学“中央高校基本科研业务费专项基金”资助项目（1zu-jbky-2011-t03）
