摘要
【目的】研究白介素(interleukin,IL)-8基因-251A/T位点多态性在中国高、低发区普通人群和胃癌(gastriccancer,GC)患者中的分布,并探讨其基因多态性与我国胃癌的关系。【方法】用聚合酶链反应-反向杂交法(polymerasechainreactionandreversedotblot,PCR-RDB)检测我国胃癌低发区(广东省)104例健康人和104例胃癌患者和胃癌高发区(陕西省)102例健康人和102例胃癌患者的IL-8基因-251A/T位点多态性。【结果】在胃癌低发区,胃癌患者IL-8-251A/A基因型的频率略高于正常对照组,但未达统计学意义(27.9%vs21.2%,χ2=1.27,P>0.05),胃癌患者携带IL-8-251A/A基因型不增加Hp感染后胃癌发生的危险性(χ2=1.40,P>0.05),而在胃癌高发区,胃癌患者携带IL-8-251A/A的频率明显高于普通人群(31.2%vs16.7%,χ2=6.04,P<0.05,OR=2.29,95%CI=1.17-4.46),并增加Hp感染后胃癌发生的危险性(χ2=6.38,P<0.05,OR=4.71,95%CI=1.36-16.30)。【结论】IL-8-251A等位基因与我国汉族人群胃癌的发生相关,以高发区明显。
[Objective] To study the relationship among interleukin 8 gene -251 locus polymorphism, Helicobacter pylori infection, and gastric cancer in high prevalent （Shanxi province） and low prevalent （Guangdong province） regions in China. [Methods] Genomic DNA was extracted from the peripheral blood of 104 healthy controls and 104 gastric patients from Guangdong, and 102 healthy controls and 102 gastric cancer patients from Shanxi. Polymorphism of IL-8-251 locus was analyzed by polymemse chain reaction and reverse dot blot （PCR- RDB）. [Results] In the low prevalence region, the frequency of IL-8-251 A/A locus of the patients with gastric cancer was slightly higher than that of healthy controls （26.0 % vs 21.2 %, χ^2=1.27, P 〉0.05）. Hp infection combined with IL-8-251 A/A locus in those patients had no effect on the risk of gastric cancer development （χ^2= 1.40, P 〉0.05）. In high prevalence region, the number of patients carrying with IL-8-251 MA is much higher than those of healthy controls （31.2% vs 16.7%,χ^2= 6.04, P〈 0.05, OR = 2.29, 95% CI = 1.17-4.46）, IL-8-251 A/A can significantly increase the risk of gastric cancer occurrence after Hp infection （χ^2=6.38, P〈 0.05, OR=4.71, 95 %CI= 1.36-16.30）. [Conclusion] IL-8-251A locus is associated with gastric caner in China, especially in high prevalence region.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2005年第5期537-540,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省医学科研基金资助项目(420002176)
关键词
白介素8
基因多态性
胃癌
interleukin 8
polymorphism
gastric cancer
