摘要
用水合联肼作还原剂研制成亲水纳米铜颗粒 ,用琥珀酸二异辛酯磺酸钠 /丙三醇 /正庚烷反胶束体系合成出憎水纳米铜颗粒 ,并通过透射电镜和紫外光谱考察了制得的纳米颗粒样品 .用憎水纳米铜颗粒及亲水纳米铜颗粒与聚乙烯醇缩丁醛构成复合固酶膜基质 ,用溶胶 凝胶法固定葡萄糖氧化酶 ,构建葡萄糖生物传感器 .实验结果表明 ,纳米铜颗粒可大幅度提高固定化酶的催化活性 ,响应电流从相应浓度的几十纳安增强到几千纳安 .从理论和实验上证明了纳米颗粒对固定酶的作用 ,讨论了纳米颗粒对酶催化性能的改善作用 。
Hydrophilic copper particles were prepared by using hydrazine as reductant and hydrophobic copper particles were produced in AOT (Aerosol OT,sodium bis(2 ethylhexyl)sulfosuccinate) glycerin heptane reverse micellar system. Characterized with the transmission electron microscope and the absorption spectra, the properties of the obtained nano copper particles were studied. Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix, which is composed of hydrophilic copper, or hydrophobic copper, and polyvinyl butyral(PVB) by a sol gel method. The results of the experiments showed that the nano copper particles can significantly enhance the catalytic activity of the immobilization enzyme, and the current response is increased by hundreds times of nanoampere for the same glucose concentration. The experiments and theory proved the function of nano particles in immobilization of enzyme. The catalysis of enzyme improved by nanoparticles was discussed. This work provides experimental and theoretical basis for preparation of useful glucose biosensor.
出处
《催化学报》
SCIE
CAS
CSCD
北大核心
2000年第5期455-458,共4页
基金
国家自然科学基金!(6 97310 10
6 99710 2 3
2 98810 0 1) .
关键词
纳米铜颗粒
葡萄糖生物传感器
糖尿病
诊断
nano copper particle, immobilization enzyme, glucose oxidase, biosensor
