摘要
SDS-PAGE研究丝素蛋白的组成于同隐,蔡再生,黄伟达(复旦大学高分子科学研究所,生物化学系,上海,200433)关键词丝素蛋白,亚基组成,聚丙烯酰胺凝胶,电泳由于丝素蛋白结构的复杂性及其易变性、水解等原因,致使研究结果往往互不相同。Tashiro...
The fibroin proteins directly extracted from silk glands of Bombyx mori were used for polyacrylamide gel electrophoresis in th presence of sodium dodecyl sulfate (SDS-PAGE). The subunit structure of fibroin molecule was discussed. It has been elucidated that the native fibroin molecule consists of subunits a,b and c with molecular weights of 280 kD, 230 kD and 25 kD, respectively, Since the amount of them is much more than that of the b subunit, the a and b subunits are the major component of fibroin. Of which the b subunit is composed of smaller e subunit of M_w about 130 kD and f subunit of M_W about 125 kD,linked by disulfide bonds.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1996年第5期829-831,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
国家博士后基金
上海市博士后基金