摘要
目的:通过观察治疗前后基质金属蛋白酶及其抑制剂的变化,探讨加味四妙散防治膝骨关节炎的可能机制。方法:60例膝骨关节炎患者,予加味四妙散免煎颗粒剂干预8周,应用ELISA技术、Real-time PCR技术检测治疗前后基质金属蛋白酶(MMPs-1)及其抑制剂(TIMPs-1)的变化。结果:治疗前,KOA患者MMPs-1含量及其mRNA变化呈上升趋势,TIMPs-1含量及其mRNA变化呈下降趋势;经予加味四妙散干预8周后,MMPs-1含量及其mRNA变化呈下降趋势,较治疗前比较有统计学差异(P<0.05);但是治疗后TIMPs-1含量及其mRNA变化与治疗前比较,无统计学差异(P>0.05)。结论:加味四妙散可能通过下调MMPs-1表达,发挥缓解膝骨关节炎症状的效用。
Objective:To explore the mechanism of jiawei simiao powder on knee osteoarthritis by observing the changes of matrix metalloproteinase 1(MMPs-1) and matrix metalloproteinase inhibitor 1(TIMPs-1) before and after treatment.Methods:60 cases with knee osteoarthritis were treated with jiawei simiao powder for 8 weeks,the contents of serum MMPs-1 and TIMPs-1 before and after treatment were determined by ELISA and MMPs-1 mRNA and TIMPs-1 mRNA were determined by Real time PCR.Results:MMPs-1 content and its mRNA expression were increased and TIMPs-1 content and mRNA expression were decreased in patients with knee osteoarthritis,MMPs-1 content and its mRNA expression were obviously reduced after treated with jiawei simiao powder for 8 weeks,there was significant difference between before and after treatment(P < 0.05),but the changes of TIMPs-1 content and mRNA expression were not obviously after treated with jiawei simiao powder for 8 weeks,there was no significant difference before and after treatment.(P > 0.05) .Conclusion:Jiawei simiao powder could ease the symptoms of knee osteoarthritis patients by down regulating MMPs-1 expression.
出处
《中国中医药科技》
CAS
2013年第4期336-337,327,共2页
Chinese Journal of Traditional Medical Science and Technology
