摘要
目的探索从冰川地区分离和培养低温菌噬菌体,并对其特征进行研究。方法利用"双层平板法"从明永冰川地区分离得到一株蜡样芽孢杆菌烈性低温噬菌体(命名为MYPB41-22),并对其生物学特征进行研究。结果宿主菌MYB41-22鉴定为Bacillus cereus菌株。噬菌体MYPB41-22在电镜下呈长六棱柱状,具一短直尾和一"衣领"状结构,无鞘。"衣领"状结构由对称的2～4个短棒状结构组成,头部具多面体立体对称结构,长55 nm,宽40 nm;尾管长约40 nm;4℃时具侵染活性,在4～37℃均可产生边缘清晰透明的噬菌斑,最佳感染温度为15℃,最适感染pH为8,最适感染复数0.1,对氯仿不敏感,基因组为双链DNA,大小约20kb。结论衣壳蛋白分析表明MYPB41-22在52 kDa处主条带含量较高,推测可能为其重要的结构蛋白。
Objective To explore the characteristics of a low-temperature bacteriophage isolated from alpine glacier region.Methods A low temperature phage of Bacillus cereus named MYPB41-22 was isolated from Mingyong glacier region by ＂double plate method＂ and its biological characteristics were studied.Results Its host bacterium MYB41-22 was identified as B.cereus.The MYPB41-22 was in a shape of long and hexagonal prism,with a short straight tail and a ＂collar＂-like structure with no sheath under electron microscope.The ＂collar＂-like structure was composed of 2 to 4 symmetrical short rods,and its head was symmetrical polyhedral three-dimensional structure,55 nm long and 40 nm wide;the tail pipe was about 40 nm long.The bacteriophage could produce transparent plaques with clear and sharp edges in a temperature range of 4 to 37 ℃,and showed good infective activity at 4 ℃;The optimum temperature of infection was 15 ℃,the optimal pH of infection was 8,and the optimum multiplicity of infection was 0.1.It was insensitive to chloroform,and its genome was consisted of double-stranded DNA with the size of about 20 kb.Conclusion Capsid protein analysis showed a higher level of major protein band at 52 kDa,which was presumed to be a critical structural protein of MYPB41-22.
出处
《中国微生态学杂志》
CAS
CSCD
2013年第7期745-749,共5页
Chinese Journal of Microecology
基金
国家自然科学基金(30960079
40961001)
云南省后备人才基金项目(2009CI027)
中国科学院微生物研究所微生物资源前期开发国家重点实验室开放课题(SKLMR-20110606)
关键词
明永冰川
低温噬菌体
蜡样芽胞杆菌
分离
特征
Mingyong glacier
Cold-active bateriophage
Bacillus cereus
Isolation
Characterization
