摘要
目的研究Wnt信号通路的核心因子β连环蛋白(β-catenin)在大鼠酒精性肝纤维化(AHF)中的表达及意义。方法采用白酒辅以玉米油、吡唑混合食料灌胃的方法制备大鼠AHF模型,于造模12 w后,采用Masson染色法观察肝组织纤维化程度,采用Elisa法检测大鼠血清层粘连蛋白(LN)、Ⅲ型胶原前蛋白(PⅢP)水平,逆转录聚合酶链反应(RT-PCR)检测大鼠肝组织β-catenin mRNA的表达。结果正常肝组织中有少量β-catenin表达,12 w末模型组大鼠肝组织中β-catenin表达明显升高。β-catenin的表达量与肝组织纤维化程度,以及血清LN、PⅢP水平呈正相关(r=0.926,0.893,0.902,P<0.01)。结论 Wnt信号通路在AHF发生发展过程中可能发挥了重要的作用。
Objective To explore the expression and significance of β-actenin（the key factor of Wnt signaling pathway） in rat models of alcoholic hepatic fibrosis.Methods 60 Wistar rats were divided into two groups at random： normal group（n=30）,model group（n=30）.Rat model of alcohol hepatic fibrosis was induced by perfusing stomach with the mixture of liquor,pyrazol and corn oil.After 12 weeks intervention,variety index of hepatic fibrosis and expression of β-catenin mRNA in liver tissue were observed.Results Compared with normal rats,the degree of hepatic fibrosis,the level of PⅢP,LN in blood serum and the expression of β-catenin mRNA were increased in model rats.The expression of β-catenin and the degree of hepatic fibrosis,the level of PⅢP,LN in blood serum were positively correlative（r=0.926,0.893,0.902,P0.01）.Conclusions Wnt signaling pathway may be involved in the genesis of alcoholic hepatic fibrosis and be closely related to the development of alcoholic hepatic fibrosis.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2013年第13期3097-3098,共2页
Chinese Journal of Gerontology
基金
天津市卫生局科技基金项目(No.2010KZ119)
天津市高等学校科技发展基金计划项目(No.20110210)
