摘要
目的检测单核细胞趋化蛋白-1（MCP-1）在急性前葡萄膜炎（AAU）及小柳原田综合征（VKH）患者血清中的表达。探讨其在葡萄膜炎发病中的可能机制。方法收集62例葡萄膜炎患者（AAU患者43例、VKH患者19例），14例正常对照组，提取血清。将AAU患者分为HLA—B27阳性组17例和HLA—B27阴性组26例；并将VKH患者就诊时是否已使用糖皮质激素治疗分为未使用激素组11例和已使用激素组8例。采用ELISA法检测各组血清中MCP-1的含量，对其进行统计分析。结果AAU患者HLA—B27阳性组与阴性组血清中MCP-1浓度分别为（115．220±22．698）pg／ml、（102．210±38．689）pg／ml，与正常对照组血清MCP-1浓度（107．900±21．655）pg／ml比较差异无统计学意义（P〉0．05）。未使用激素治疗的VKH患者外周血清中MCP-1含量（217．370±38．751）pg／ml较正常组显著增高（P〈0．05）。激素治疗VKH组MCP-1含量（195．480±19．977）pg／ml略低于未治疗组（P〉0．05），但仍高于正常组（P〈0．05）。结论与正常对照组比较，AAU患者血清MCP-1的表达无明显改变；而VKH患者血清MCP-1的表达上调，推测其可能参与了VKH的病理生理过程，全身使用糖皮质激素可能通过抑制MCP-1表达控制眼部炎症。
Objective To explore the effect of monocyte chemoattractant protein-1 （MCP-1） in uveitis pathogenetic mechanism by detecting the concentration of MCP-1 in serum of AAU and VKH. Methods Sixty-two patients suffered uveitis were collected （43 AAU patients, 19 VKH pa- tients）, and 14 healthy individuals served as control group. Patients with AAU were divided into HLA-B27 （＋） group and HLA-B27 （-） group. Meanwhile, patients with VKH were divided into two groups according to whether steroid was administered systematically or not （VKH patients who had administered steroid systematically （group A）; patients without any systemic therapy of steroid （group B）. The level of serum MCP-1 was detected by ELISA. Statistic analysis was performed to compare the difference between groups. Results Serum concentration of MCP-1 in HLA-B27 positive AAU, HLA-B27 negative AAU and control group were 115.220±22.698 pg/ml, 102.210±38.689 pg/ml, 107.900±21.655 pg/ml respectively, there were no significant difference among three groups （P 〉 0.05）. The concentration of MCP-1 in VKH group B （217.370±38.751 pg/ml） was much higher than that of control group （P 〉0.05）. The concentration of MCP-1 in VKH group A （195.480±19.977 pg/ml） decreased slightly when compared with group B, but still higher than that of control group （P 〉 0.05）, Conclusions There is no change of the expression of MCP-1 in AAU. While the increased MCP-1 in VKH patient serum may participate in the pathogenesis of VKH, and steroid may sup- press ocular inflammation by inhibiting the production of MCP-1.
出处
《中国实用眼科杂志》
CSCD
北大核心
2013年第7期880-883,共4页
Chinese Journal of Practical Ophthalmology
基金
国家十一五科技支撑计划项目（2007BA118810）
