摘要
目的对当归四逆汤加味花椒的药理作用进行研究,探讨其预防治疗冻疮的作用。方法分别采用水煎和渗漉两种中药提取方法,于60℃减压浓缩制备中药提取物浸膏。将30只SD大鼠随机分为空白组、模型组、水煎组、渗漉组4组,采用冻疮治疗前后外观描述、抗炎-肿胀度检查法、镇痛作用测定、组织切片等方法来综合评价两种中药提取物的冻疮防治效果。结果当归四逆汤加味花椒的水煎、渗漉提取物均可显著减轻大鼠冻伤肿胀;可提高热板致痛小鼠在给药后0.5 h痛阈值;肉眼、镜下观察均有肿胀消退,且组织切片中皮下组织血管减少,并发现毛囊。结论当归四逆汤加味花椒具有较好的抗炎、镇痛、活血化瘀等作用,水煎和渗滤组均具有较好的冻疮防治效果,对实验性动物冻伤后皮肤具有一定的促进康复作用。
Objective To study the pharmacological effects of modified Dangguisini decoction by adding pepper in the prevention and treatment of frostbite.Methods The water-decoction and the diacolation Methods were adopted.The Chinese herb extract was prepared by the vacuum concentration at 60 ℃.Thirty SD rats were randomly assigned into 4 groups,blank group,model group,decoction group and diacolation group.The appearance description of frostbite before and after treatment,swelling test for anti-inflammatory,analgesic effect measurement,tissue sections and other Methods were adopted to comprehensively evaluate the frostbite-preventing effects of the two kinds of traditional Chinese medicine extracts.Results Both the decoction and percolation extracts of modified Dangguisini Decoction adding pepper could significantly reduce the rat frostbite swelling,enhance the pain threshold value at 0.5 h after drug administration in the hot-plate-induced pain mice;the swelling fadeaway was found under the visual inspection or microscope observation.The tissue sections showed that the subcutaneous tissue vessels were reduced.Hair follicles were found.Conclusion Modified Dangguisini Decoction adding pepper has the better effects of anti-inflammatory,analgesic and blood-activiating and stasis-dissolving.Its extract by waterdecoction and diacolation has better effect for preventing and treating frostbite and certain promoting rehabilitation effect on the frostbite skin in the experimental animal.
出处
《中国药业》
CAS
2013年第14期36-38,共3页
China Pharmaceuticals
基金
上海市教委重点学科基金项目
项目编号:J50302
关键词
当归四逆汤
花椒
冻疮
药理作用
Dangguisini Decoction
pepper
frostbite
pharmacological effects
