摘要
目的:探讨"消肿止痛汤"通过调控创伤后大鼠炎症因子调控水平预防深静脉血栓形成的机理。方法:SPF级大鼠80只随机分成正常N组、空白B组、中药C组、西药W组,后3组再各分三小组,每小组8只。N组不做任何干预措施,余3组以"定量击打+石膏固定"法建立创伤性深静脉血栓形成模型,C组造模前1天起每日予消肿止痛汤灌胃,W组予低分子肝素皮下注射,B组不予药物干预。分别于造模后第1、3、7天处死(B1、W1、C1)组、(B2、W2、C2)组、(N、B3、W3、C3)组,心脏取血检测TNF-α、IL-1、IL-6,取下肢静脉标本观察静脉血栓发生情况。结果:造模后1天3组大鼠血清TNF-α、IL-1、IL-6水平均高于N组,差异有显著性意义(P<0.05),各组间差异无统计学意义(P﹥0.05);造模后3天,3组上述因子水平进一步升高(P<0.05),两两比较B1组明显高于C2、W2组(P<0.05),后2组间无明显差异(P>0.05);造模后7天,C3、W3组各因子较造模后3天时明显下降,差异有显著性意义(P<0.05),B3组则未见明显下降(P>0.05),两两比较B3组明显高于C3、W3组,后2组间无明显差异(P﹥0.05)。B组12例DVT(B1组1例、B2组4例、B3组7例),C组3例DVT(C1组0例、C2组1例、C3组2例),W组4例DVT(W1组0例、W2组1例、W3组3例),造模后第7天DVT发生率比较,差异有显著性意义(P<0.05)。结论:炎症反应在大鼠创伤性深静脉血栓形成中起重要作用,中药消肿止痛汤可显著的降低创伤后炎症因子水平,有效预防创伤性DVT的发生。
Objective To study the effect of XiaoZhongZhiTong decoction on regulating inflammatory cytokines levels and preventing traumatic deep venous thrombosis of rats.Methods 80 SPF rats were randomly and evenly divided into four groups： normal,blank,western medicine and chinese herb medicine.Rats in N-group did not received any intervention,while the others were established TDVT models by the means of＂beating on the bilateral thigh quantitative and plaster fixation＂.Rats in B-group did not received any medicine,rats in C-group were intragastric administrated with＂XiaoZhongZhiTong＂,rats in W-group were injected with low molecular weight heparin.The preventive effect and the change of inflammatory cytokines levels were compared in the different groups.Results ①Comparison of inflammatory cytokines levels： All rats＇ inflammatory cytokines levels increased significantly the day after model establishment（P〈0.05）.The increased inflammatory cytokines levels of the W-group and C-group decreased remarkably after treatment（P〈0.05）,while those of the B-group hardly reduced（P〈0.05）,with significant difference between the 3 groups（P〈0.05.②Comparison of TDVT incidence rates： There was statistical difference among the 3 groups at 7th day after model establishment（P ﹤ 0.05）.Conclusion Inflammatory cytokines levels are correlated with TDVT incidence rate of rats.XiaoZhongZhiTong decoction is effective on decreasing these inflammatory cytokines levels and preventing TDVT after trauma.
出处
《中国民族民间医药》
2013年第13期20-22,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
(广东省中医药管理局)中药对创伤后炎症反应调控及深静脉血栓预防机制的研究(编号:2010350)
关键词
创伤
深静脉血栓形成
预防
炎症因子
消肿止痛汤
Trauma
Deep venous thrombosis /prevention and control
Inflammatory cytokine
Animal experimentation
XiaoZhongZhiTong decoction
