摘要
目的观察巴戟天含药血清对原代破骨细胞RANK和CA Ⅱ mRNA表达的影响。方法取SPF级大鼠48只,随机分成正常组12只和去势组36只,正常组切去卵巢周围部分脂肪,去势组切除卵巢。3个月后测定两组雌激素水平并将去势组随机分为骨质疏松组,骨质疏松+雌激素组,骨质疏松+巴戟天含药血清组。采用机械分离法提取各组的破骨细胞,培养5 d后,TRAP染色及电镜扫描骨片等的方法鉴定破骨细胞。最后用雌激素或巴戟天含药血清干预3 d,以RT-PCR法检测各组RANK和CA ⅡmRNA表达。采用单因素方差分析或多样本均数两两比较进行统计分析。结果去势组后大鼠雌激素水平低于正常组(P<0.01)。骨质疏松组破骨细胞RANK和CA Ⅱ表达均高于正常组(P<0.05,P<0.05);巴戟天含药血清可降低骨质疏松后大鼠破骨细胞RANK和CA II的表达(P<0.05,P<0.05)。结论巴戟天和雌激素均可降低骨质疏松大鼠破骨细胞RANK和CA Ⅱ的表达,从而达到抑制骨质疏松的作用。
Objective To observe the effect of morinda officinalis-containing serum on the mRNA expression of RANK and CAII in primary osteoclasts in ovariectomized rats. Methods Forty-eight female SPF rats were randomly divided into 2 groups： SHAM group （ n = 12） and OVX group （n = 36）. Rats in SHAM group received the operation in which retroperitoneal fat with the same mass as bilateral ovaries was removed. And rats in OVX group were bilaterally ovarieetomized. The serum levels of estradiol （E2） of rats in both groups were detected 3 months after the operation. And the rats in OVX group were randomly divided into 3 groups： ostcoporosis （OP） group, OP ＋ E2 group, and OP ＋ RMO group. Osteoclasts in each group were collected using mechanical separation method. After 5-day culturing, the osteoclasts were identified using TRAP staining and scanning electron microscope. Finally, the osteoclasts were treated with RMO or E2 for 3 days, then the mRNA expression of RANK and CAll was detected using RT-PCR. One-way ANOVA and the LSD multiple comparison were performed. Results The serum levels of E2 in OVX group were lower than those in SHAM group （P 〈 0. 01 ）. The mRNA expression of RANK and CAll in osteoclasts in OP group was higher than that in SHAM group （P 〈0. 05 ,P 〈 0. 05, respectively）. The mRNA expression of RANK and CAⅡ in osteoclasts treated with RMO containing serum was lower than that in SHAM group （ P 〈 0. 05, P 〈 0. 05, respectively）. Conclusion Both morinda officinalisa and E2 can decrease the mRNA expression of RANK and CAⅡ, which can exert the function of inhibiting OP.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2013年第5期469-475,共7页
Chinese Journal of Osteoporosis
基金
厦门市科技局资助项目(3502Z20104031)
